EFFECTS OF TWO HERBICIDES, FLUAZYFOP-P-BUTYL AND FENOXAPROP-P-ETHYL, ON GENOTOXICITY IN DROSOPHILA SMART ASSAY AND ON PROLIFERATION AND VIABILITY OF HEK293 CELLS FROM THE PERSPECTIVE OF CARCINOGENESIS

Karadeniz A., KAYA B., Savaş B., TOPCUOĞLU Ş. F.

FRESENIUS ENVIRONMENTAL BULLETIN, cilt.24, sa.6B, ss.2052-2056, 2015 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 24 Sayı: 6B
  • Basım Tarihi: 2015
  • Dergi Adı: FRESENIUS ENVIRONMENTAL BULLETIN
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.2052-2056
  • Anahtar Kelimeler: Drosophila melanogaster, wing spot test, fluazifop-p-butyl, fenoxaprop-p-ethyl, genotoxic effect, cancer proliferation, WING-SPOT-TEST, RECOMBINATION TEST, SOMATIC MUTATION, MELANOGASTER, TOXICITY
  • Akdeniz Üniversitesi Adresli: Evet

Özet

In this study, the mutagenic and recombinogenic effects of herbicides, fluazifop-p-butyl (FPB) and fenoxaprop-p-ethyl (FPE), widely used in the agricultural regions, were investigated using the somatic mutation and recombination test (SMART) in Drosophila wings. The viability and proliferation effects were tested on human immortalized embryonic kidney HEK293 cells, and were also examined with MTT and trypan-blue exclusion assays, at the early stage of carcinogenesis. For the SMART assay, two different crosses were used: a standard (ST) and a high-bioactivation (HB) cross, involving the flare-3 (flr(3)) and the multiple wing hairs (mwh) markers. The HB cross involved flies characterized by an increased cytochrome P-450-dependent bioactivation capacity, which permits the more efficient biotransformation of promutagens and procarcinogens. There is not any positive result in both ST and HB crosses of Drosophila. Furthermore, both herbicides affected the proliferation rate of the HEK293 cells but both induced cell death at high concentrations.