Dimensional assessment of X-bearing and Y-bearing haploid and disomic human sperm with the use of fluorescence in situ hybridization and objective morphometry
FERTILITY AND STERILITY, vol.85, no.1, pp.121-127, 2006 (SCI-Expanded, Scopus)
- Publication Type: Article / Article
- Volume: 85 Issue: 1
- Publication Date: 2006
- Doi Number: 10.1016/j.fertnstert.2005.07.1295
- Journal Name: FERTILITY AND STERILITY
- Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
- Page Numbers: pp.121-127
- Keywords: sperm, X-bearing spermatozoa, Y-bearing spermatozoa, dimensional attributes, haploid, dosomic, HUMAN SPERMATOZOA, DIMINISHED-MATURITY, ANEUPLOIDY, SHAPE, FREQUENCIES, ABSENCE
- Akdeniz University Affiliated: Yes
Abstract
Objective: To explore the dimensional attributes of haploid and disomic X-bearing and Y-bearing spermatozoa.
Design: Morphometric evaluation of more than 2,000 X-bearing and Y-bearing spermatozoa after identification of the genotype with fluorescence in situhybridization.
Setting: Academic clinical and research andrology laboratory.
Main Outcome Measure(s): Sperm head area, perimeter, long axis, short axis, shape factor, elliptical form factor (long axis/short axis), and tail length.
Result(s): We found no differences in dimensions or dimensional distributions between X-bearing and Y-bearing spermatozoa, whether in the native or thedecondensed state, or in oligozoospermic or normozoospermic men. There were inconsistent differences and a 70% overlap in the dimensions of haploid anddisomic spermatozoa. The other 30% of sperm with disomic nuclei were either smaller or larger compared to haploid spermatozoa.
Conclusion(s) There are no differences, or distinguishing characteristics, in dimensions or dimensional distributions between X-bearing and Y-bearingspermatozoa. Dimensional attributes do not discriminate between dysomic and haploid spermatozoa.