Dimensional assessment of X-bearing and Y-bearing haploid and disomic human sperm with the use of fluorescence in situ hybridization and objective morphometry

Zavaczki Z., Celik-Ozenci C., Ovari L., Jakab A., Sati G. L., Ward D., ...Daha Fazla

FERTILITY AND STERILITY, cilt.85, sa.1, ss.121-127, 2006 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 85 Sayı: 1
  • Basım Tarihi: 2006
  • Doi Numarası: 10.1016/j.fertnstert.2005.07.1295
  • Dergi Adı: FERTILITY AND STERILITY
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.121-127
  • Anahtar Kelimeler: sperm, X-bearing spermatozoa, Y-bearing spermatozoa, dimensional attributes, haploid, dosomic, HUMAN SPERMATOZOA, DIMINISHED-MATURITY, ANEUPLOIDY, SHAPE, FREQUENCIES, ABSENCE
  • Akdeniz Üniversitesi Adresli: Evet

Özet

Objective: To explore the dimensional attributes of haploid and disomic X-bearing and Y-bearing spermatozoa.

Objective: To explore the dimensional attributes of haploid and disomic X-bearing and Y-bearing spermatozoa. 

Design: Morphometric evaluation of more than 2,000 X-bearing and Y-bearing spermatozoa after identification of the genotype with fluorescence in situhybridization. 

Setting: Academic clinical and research andrology laboratory. 

Main Outcome Measure(s): Sperm head area, perimeter, long axis, short axis, shape factor, elliptical form factor (long axis/short axis), and tail length. 

Result(s): We found no differences in dimensions or dimensional distributions between X-bearing and Y-bearing spermatozoa, whether in the native or thedecondensed state, or in oligozoospermic or normozoospermic men. There were inconsistent differences and a 70% overlap in the dimensions of haploid anddisomic spermatozoa. The other 30% of sperm with disomic nuclei were either smaller or larger compared to haploid spermatozoa. 

Conclusion(s) There are no differences, or distinguishing characteristics, in dimensions or dimensional distributions between X-bearing and Y-bearingspermatozoa. Dimensional attributes do not discriminate between dysomic and haploid spermatozoa.