Alterations in the erythrocyte antioxidant system of blood stored in blood bags
Research Communications in Molecular Pathology and Pharmacology, vol.109, no.5-6, pp.357-363, 2001 (SCI-Expanded, Scopus)
- Publication Type: Article / Article
- Volume: 109 Issue: 5-6
- Publication Date: 2001
- Journal Name: Research Communications in Molecular Pathology and Pharmacology
- Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
- Page Numbers: pp.357-363
- Keywords: Antioxidant activity, Catalase (CAT), Erythrocyte storage, Glucose-6-phosphate dehydrogenase (G-6-PD), Glutathione (GSH), Glutathione peroxidase (GSH-Px), Glutathione S-transferase (GSH-S-T), Lipid peroxidation, Malonyldialdehyde (MDA)
- Akdeniz University Affiliated: Yes
Abstract
In the present study, we measured the concentrations of reduced glutathione (GSH) and malonyldialdehyde (MDA) and the activities of glutathione peroxidase (GSH-Px), glutathione S-transferase (GSH-S-T), superoxide dismutase (SOD), catalase (CAT) and glucose-6-phosphate dehydrogenase (G-6-PD) in erythrocytes obtained freshly from adult male donors which was preserved with CPDA-1 anticoagulant (citrate, phosphate, dextrose, adenine) on different days of storage. At the end of the study, storage-associated alterations in antioxidant activities were noted and discussed. GSH, GSH-Px, GSH-S-T, SOD, CAT and G-6-PD activities decreased, but erythrocyte MDA levels, as an index of lipid peroxidation, increased during the storage period. According to our results, glutathione-dependent antioxidant systems in erythrocytes might be depleted during long storage in blood bags.