Alterations in the erythrocyte antioxidant system of blood stored in blood bags

KORGUN D. K., BİLMEN S., YEŞİLKAYA A.

Research Communications in Molecular Pathology and Pharmacology, vol.109, no.5-6, pp.357-363, 2001 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Article
  • Volume: 109 Issue: 5-6
  • Publication Date: 2001
  • Journal Name: Research Communications in Molecular Pathology and Pharmacology
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.357-363
  • Keywords: Antioxidant activity, Catalase (CAT), Erythrocyte storage, Glucose-6-phosphate dehydrogenase (G-6-PD), Glutathione (GSH), Glutathione peroxidase (GSH-Px), Glutathione S-transferase (GSH-S-T), Lipid peroxidation, Malonyldialdehyde (MDA)
  • Akdeniz University Affiliated: Yes

Abstract

In the present study, we measured the concentrations of reduced glutathione (GSH) and malonyldialdehyde (MDA) and the activities of glutathione peroxidase (GSH-Px), glutathione S-transferase (GSH-S-T), superoxide dismutase (SOD), catalase (CAT) and glucose-6-phosphate dehydrogenase (G-6-PD) in erythrocytes obtained freshly from adult male donors which was preserved with CPDA-1 anticoagulant (citrate, phosphate, dextrose, adenine) on different days of storage. At the end of the study, storage-associated alterations in antioxidant activities were noted and discussed. GSH, GSH-Px, GSH-S-T, SOD, CAT and G-6-PD activities decreased, but erythrocyte MDA levels, as an index of lipid peroxidation, increased during the storage period. According to our results, glutathione-dependent antioxidant systems in erythrocytes might be depleted during long storage in blood bags.