When susceptible cultivars are grown in calcareous soils with high pH, significant yield loss due to iron (Fe) deficiency clalorosis is brought about in chickpea (Cicer arietinum L.). One of the most efficient ways for solve this problem is improved of Fe-deficiency chlorosis via conventional breeding methods. In the study, genotypes ICC 4851 and ICC 4858, which are resistant to Fe-deficiency chlorosis, were crossed with genotype ICC 6119, which is susceptible to Fe-deficiency chlorosis, and studied genetics of Fe-deficiency chlorosis in F-1 and F-2 segregating generations. Fe-deficiency chlorosis was governed by a major recessive gene and affected by environment factors like high temperature. A negative selection seems to be an effective approach after segregation in F-2 or later generations.