Antibacterial transparent thin films, containing different amounts of silver ion, have been prepared on a glass substrate by the sol-gel process. Thin films were obtained from inorganic-organic hybrid sols derived from 3-(glicydiloxypropyl)trimethoxy silane, N-(2-aminoethyl)-3-aminopropyl-trimethoxysilane and aluminium-sec-butoxide/ethylaceto-acetate complex and doped with silver ions. The films were characterized by Scanning Electron Microscopy and Raman Spectroscopy. The physical properties of the films were studied by solar-box, taber abraser, hardness test pencil, scratch-adhesion test and spectroscopy. The antibacterial activity of the coatings were investigated against gram negative Escherichia coli and gram positive Staphylococcus aureus. The results showed that 1% doping of the transparent hybrid thin film with silver ions resulted in high antibacterial properties of the film.