Experimental hyperhomocysteinemia disturbs bone metabolism in rats

Ozdem S., Samanci N., Tasatargil A., Yildiz A., Sadan G., Donmez L., ...Daha Fazla

SCANDINAVIAN JOURNAL OF CLINICAL & LABORATORY INVESTIGATION, cilt.67, sa.7, ss.748-756, 2007 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 67 Sayı: 7
  • Basım Tarihi: 2007
  • Doi Numarası: 10.1080/00365510701342088
  • Dergi Adı: SCANDINAVIAN JOURNAL OF CLINICAL & LABORATORY INVESTIGATION
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.748-756
  • Anahtar Kelimeler: biological markers, bone, bone density, homocysteine, hyperhomocysteinemia, osteoporosis, rats, COLLAGEN CROSS-LINKING, MINERAL DENSITY, TURNOVER MARKERS, METHIONINE LOAD, HIP-FRACTURES, HOMOCYSTEINE, FOLATE, VITAMIN-B-12, POLYMORPHISM, ASSOCIATION
  • Akdeniz Üniversitesi Adresli: Evet

Özet

Objective. To investigate whether experimental hyperhomocysteinemia (HHCY) can induce adverse changes in bone metabolism. Methods. Blood and urine samples were collected from rats fed with a methionine-enriched diet (HHCY, n=18) or an isocaloric control diet (control, n=10) for 12 weeks. Biochemical bone turnover markers (osteocalcin, hydroxyproline, N-terminal collagen I telopeptides and homocysteine (HCY), folate and vitamin B12) were measured. Whole body bone mineral density (BMD) was assessed by dual energy X-ray absorptiometry. Results. HCY was significantly higher in HHCY than in control rats (16.2 versus 3.2 mu mol/L; p=0.0006). Bone resorption parameters hydroxyproline (1.60 +/- 0.6 versus 0.85 +/- 0.4; p < 0.05) and N-terminal collagen I telopeptides (150.8 +/- 78 versus 48.1 +/- 26 nmol/L BCE; p < 0.05) increased, whereas bone formation marker osteocalcin (9.01 +/- 3.8 versus 15.07 +/- 4.2 ng/mL; p < 0.05) decreased in HHCY compared to control rats. The relation N-terminal collagen I telopeptides/osteocalcin significantly increased in HHCY compared to control rats (13.14 +/- 3.1 versus 4.14 +/- 1.9). BMD measurement did not reveal any differences between groups. Conclusion. These findings demonstrate a significant modification of bone turnover in HHCY rats. The relation between bone resorption and formation indicates a shift toward bone resorption, which might be a plausible explanation for the relation between HHCY and fracture risk.