CAPS-microsatellites: use of CAPS method to convert non-polymorphic microsatellites into useful markers

İNCE A. G., KARACA M., ONUS A. N.

MOLECULAR BREEDING, cilt.25, sa.3, ss.491-499, 2010 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 25 Sayı: 3
  • Basım Tarihi: 2010
  • Doi Numarası: 10.1007/s11032-009-9347-y
  • Dergi Adı: MOLECULAR BREEDING
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.491-499
  • Anahtar Kelimeler: Capsicum, Gossypium, Co-dominant marker, EST, Restriction enzymes, SIMPLE SEQUENCE REPEATS, EST-SSR MARKERS, GENETIC DIVERSITY, TRANSFERABILITY, BARLEY, EVOLUTIONARY, PROGRAM, MUTANT, GENERA
  • Akdeniz Üniversitesi Adresli: Evet

Özet

Microsatellites found in expressed sequence tags (ESTs) have several advantages in comparison to conventional microsatellites developed from genomic DNA. Advantages of EST-microsatellites include better transferability between the species and lower development cost of primer pairs. However, in many cases the amplified products of genomic DNA are larger than their expected sizes based on ESTs and these markers have low level of polymorphism. In the present study we report on a new approach as a modest modification of the cleavage amplified polymorphic sequence (CAPS) called CAPS-microsatellites. Using a total of 20 EST-microsatellite primer pairs on four Capsicum species and two Gossypium species we observed that all 20 monomorphic EST-microsatellites could be converted into polymorphic markers among the species used. Further analyses using a total of 37 F-2 individuals obtained from two Capsicum annuum L. lines indicated that CAPS-microsatellite markers usually segregated as co-dominant fashions, discriminating the homozygous from heterozygous genotypes and could be used in genetic analyses of plant species.