Akademik Veri Yönetim Sistemi
BULGARIAN JOURNAL OF AGRICULTURAL SCIENCE, cilt.18, sa.5, ss.682-688, 2012 (SCI-Expanded)
Severe bacterial symptoms were observed in tomato growing areas of Tokat province, Turkey. The tomato plants have
asymmetric wilting appearing on leaves with brown discolorations of vascular systems. A total of 45 bacterial isolates were
collected from all tomato grown areas of Tokat province. A potassium hydroxide (KOH) string test was applied to all the
bacterial samples: 9 of the 45 bacterial colonies were gram positive and 36 colonies were gram negative. The gram positive
bacterial colonies were analyzed with Clavibacter michiganensis subsp. michiganensis specific primers Cmm5 and Cmm6 in
PCR assays of which 3 produced 614 bp amplification products and were identified as C. michiganensis subsp. michiganensis.
The 36 bacterial gram negative colonies were analyzed with Ralstonia ssp. specific primers RsolfliC forward and RsolfliC
reverse of which 4 colonies amplified 400 bp fragments and were identified as Ralstonia ssp. Pathogenicity tests with 7 tomato
cultivars and 10 wild tomato accessions revealed that all the 7 tomato cultivars were susceptible to virulent Cmm2 isolate.
However, 2 wild tomato accessions (LA1054, LA1318) were resistant, 6 accessions were susceptible and 2 accessions were
enhanced susceptible to the Cmm2 isolate. The results of this study demonstrated express and reliable detection methods of
bacterial canker disease in tomatoes and possible resistance sources to bacterial canker disease from wild tomato accessions.