Green synthesis of carbon based biosensor materials from algal biomass for the sensitive detection of vardenafil

Bilge S., Bakirhan N. K., DONAR Y. O., SINAĞ A., ÖZKAN S. A., OKUDAN E. Ş.

JOURNAL OF ELECTROANALYTICAL CHEMISTRY, cilt.871, 2020 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 871
  • Basım Tarihi: 2020
  • Doi Numarası: 10.1016/j.jelechem.2020.114286
  • Dergi Adı: JOURNAL OF ELECTROANALYTICAL CHEMISTRY
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, Chimica, Compendex, INSPEC
  • Anahtar Kelimeler: Sensor, Cystoseira algae, Hydrothermal carbonization, Electrooxidation, Vardenafil, HYDROTHERMAL CARBONIZATION, CATION-EXCHANGE, NANOPARTICLES, ELECTRODE
  • Akdeniz Üniversitesi Adresli: Evet

Özet

In this study, a novel carbon electrode material for improved electrochemical sensing of vardenafil (VRF) as a model drug active substance was prepared by two cycled hydrothermal carbonization (HTC) of algae-based biomass for the first time in open literature. In order to increase the electrical conductivity more, multi-walled carbon nanotube (MWCNT) was used as a catalyst during the second cycle of HTC. The surface morphology and chemical structure of the synthesized carbonaceous materials were investigated by using different techniques. The voltammetric behaviour of VRF drug was investigated on glassy carbon electrode (GCE) modified by algae based carbon electrode material. MWCNT catalyzed carbon material obtained from second cycle of HTC treatment exhibited more sensitive, reproducible and low oxidation potential comparing to bare GCE in the presence of VRF due to the increased degree of graphitization. At the optimum conditions, differential pulse voltamograms (DPV) method with MWCNT catalyzed carbon material exhibited high sensitivity with the detection limits of 96.3 pM at pH 1.0 H2SO4 solution, 60.6 nM at serum and 46.7 nM at urine samples. Finally, the proposed sensor was successfully applied in tablet formulation, human serum and human urine samples.