Akademik Veri Yönetim Sistemi
Journal of Pharmaceutical and Biomedical Analysis Open, cilt.6, 2025 (Scopus)
Caffeic acid phenethyl ester (CAPE), a key bioactive component of propolis, is recognized for its wide-ranging therapeutic properties. In this study, a robust and sensitive high-performance liquid chromatography (HPLC) method was developed and validated to quantify CAPE in propolis and honey samples collected from various regions of Turkiye. The method exhibited high linearity (R² = 0.999), precision, and sensitivity, achieving a limit of detection (LOD) of 2.70 ppm and a limit of quantification (LOQ) of 8.30 ppm. Analysis of 20 samples revealed considerable variability in CAPE content based on sample type and geographical origin. CAPE was detected in 9 of the samples, with the highest concentrations observed in propolis, reaching up to 13154.94 ppm. In contrast, most honey samples contained CAPE below the LOQ. These findings confirm that propolis serves as the primary source of CAPE among bee-derived products and underscore the impact of regional flora on its phytochemical composition of profile.