QTL analysis of Na+ and Cl- accumulation related traits in an intergeneric BC1 progeny of Citrus and Poncirus under saline and nonsaline environments

Tozlu I., Guy C., Moore G.

GENOME, cilt.42, sa.4, ss.692-705, 1999 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 42 Sayı: 4
  • Basım Tarihi: 1999
  • Doi Numarası: 10.1139/gen-42-4-692
  • Dergi Adı: GENOME
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.692-705
  • Anahtar Kelimeler: Citrus grandis, Poncirus trifoliata, salinity stress, QTL mapping, transgressive segregation, mineral analysis, sodium, chloride, salt tolerance, citrus genetics, FRAGMENT-LENGTH-POLYMORPHISMS, SALT-TOLERANCE, RAPD MARKERS, K+/NA+ DISCRIMINATION, QUANTITATIVE TRAITS, MENDELIAN FACTORS, LINKAGE MAP, TOMATO, LOCI, PLANTS
  • Akdeniz Üniversitesi Adresli: Hayır

Özet

The effects of salinization with 40 mM NaCl on Poncirus trifoliata (L.) Raf., Citrus grandis (L.) Osb., their F-1, and a BC1 progeny population (C. grandis x (F-1)) were investigated by means of Na+ and Cl- analyses and QTL (quantitative trait loci) mapping. A total of 38 traits related to different tissue or whole-plant Na+ and (or) Cl- accumulation was analyzed in salinized and nonsalinized BC1 progeny clones. The comparison of the three parental types with the BC1 progeny under control and saline conditions showed that the BC1 progeny plants segregated transgressively for many traits. First mapping analyses resulted in a total of 73 potential quantitative trait loci (PQTL) with LOD scores greater than or equal to 3.0 located on a previously generated linkage map. Fifty-three percent of the mapped PQTLs were for traits associated with salinity. The small progeny population size used made further analyses of these PQTLs necessary. By considering LOD scores, map locations, and correlation analyses of the traits, it was possible to identify 17 regions of the citrus genome of interest: 8 of them may contain genuine QTLs of large effect and 9 regions are worthy of further study. Correlation analyses and locations of PQTLs indicated that many traits were controlled by fewer genes than the actual number of QTLs mapped for them. For example, 21 PQTLs mapped for Na+ accumulation and Cl-/Na+ ratios were located in a cluster at the beginning of one linkage group (LG), while 10 PQTLs mapped for Cl- accumulation and Cl-/Na+ ratios were located in a cluster at the beginning of another LG. This is the first step in identifying QTLs that have a major impact on salt tolerance and (or) mineral accumulation in citrus.

Abstract

The effects of salinization with 40 mM NaCl on Poncirus trifoliata (L.) Raf., Citrus grandis (L.) Osb., their F-1, and a BC1 progeny population (C. grandis x (F-1)) were investigated by means of Na+ and Cl- analyses and QTL (quantitative trait loci) mapping. A total of 38 traits related to different tissue or whole-plant Na+ and (or) Cl- accumulation was analyzed in salinized and nonsalinized BC1 progeny clones. The comparison of the three parental types with the BC1 progeny under control and saline conditions showed that the BC1 progeny plants segregated transgressively for many traits. First mapping analyses resulted in a total of 73 potential quantitative trait loci (PQTL) with LOD scores greater than or equal to 3.0 located on a previously generated linkage map. Fifty-three percent of the mapped PQTLs were for traits associated with salinity. The small progeny population size used made further analyses of these PQTLs necessary. By considering LOD scores, map locations, and correlation analyses of the traits, it was possible to identify 17 regions of the citrus genome of interest: 8 of them may contain genuine QTLs of large effect and 9 regions are worthy of further study. Correlation analyses and locations of PQTLs indicated that many traits were controlled by fewer genes than the actual number of QTLs mapped for them. For example, 21 PQTLs mapped for Na+ accumulation and Cl-/Na+ ratios were located in a cluster at the beginning of one linkage group (LG), while 10 PQTLs mapped for Cl- accumulation and Cl-/Na+ ratios were located in a cluster at the beginning of another LG. This is the first step in identifying QTLs that have a major impact on salt tolerance and (or) mineral accumulation in citrus.