Akademik Veri Yönetim Sistemi
Bahçe, cilt.51, sa.1, ss.146-149, 2022 (Hakemli Dergi)
Pepper (Capsicum annuum L.) has a wide consumption on our tables, from daily fresh consumption to the food industry,
it can also be used in many different areas such as alternative treatment methods, tropical creams, aerosol sprays and
defense weapons due to its capsaicinoid content. Tomato spotted wilt virus (TSWV) is commonly encountered in the
cultivation areas of pepper, which is one of our important export items. The Tsw gene of the C.chinense species controls
resistance to TSWV in peppers and this gene is now transferred to cultivars by breeding methods. While peppers
containing the Tsw gene did not show TSWV symptoms, symptoms were observed and reported in resistant pepper
varieties in Spain in 2004, in 2014 in Samsun, and in 2016 in Antalya. In this study, local varieties found in the gene
resources of our company; Hatay, Urfa, Antep, Elazig and Maraş pepper lines were used. These pepper lines were
molecularly tested for the presence of the Tsw gene. The accuracy between molecular and classical testing will be
compared by infecting the peppers with gene screening with TSWV isolates collected in Kaş, Demre, Kumluca, Aksu,
Serik and Gazipaşa, districts of Antalya. It was amplified by PCR using molecularly Tsw gene-specific primer SCAC568,
then digested with XBAI and TaqI enzymes. Results while 2 of 70 plants were found to be homozygous and 43 of them
heterozygous resistant, 25 of them were found susceptible. It was observed that 8 days after the contamination of the
isolates collected from 6 different regions, symptoms began to occur in peppers that were found to be molecularly
resistant. The result was positive when these peppers were tested with the TSWV disease primers. It was determined that
there is TSWV 1 isolate that overcomes the resistance commonly in the regions where agriculture is made in Antalya.