Therapeutic Effects of Alpha Lipoic Acid and Vitamin C on Alveolar Bone Resorption After Experimental Peripdontitis in Rats: A Biochemical, Histochemical, and Stereologic Study

Akman S., Canakci V., Kara A., Tozoglu U., ARABACI T., Dagsuyu I. M.

JOURNAL OF PERIODONTOLOGY, cilt.84, sa.5, ss.666-674, 2013 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 84 Sayı: 5
  • Basım Tarihi: 2013
  • Doi Numarası: 10.1902/jop.2012.120252
  • Dergi Adı: JOURNAL OF PERIODONTOLOGY
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.666-674
  • Anahtar Kelimeler: Antioxidants, oxidative stress, periodontitis, periodontium, pharmaceutical preparations, OXIDATIVE STRESS, REACTIVE OXYGEN, LIPID-PEROXIDATION, RECEPTOR ACTIVATOR, ASCORBIC-ACID, IN-VITRO, INFLAMMATION, ANGIOGENESIS, ANTIOXIDANTS, NEUTROPHILS
  • Akdeniz Üniversitesi Adresli: Evet

Özet

Background: Alpha lipoic acid (ALA) and vitamin C (Vit-C) are very important and powerful antioxidants that have been used for the treatment of many diseases. The present study aims to investigate the role of ALA and Vit-C substances in the treatment of alveolar bone resorption in periodontal diseases.

Abstract

BACKGROUND:

Alpha lipoic acid (ALA) and vitamin C (Vit-C) are very important and powerful antioxidants that have been used for the treatment of many diseases. The present study aims to investigate the role of ALA and Vit-C substances in the treatment of alveolar bone resorption in periodontal diseases.

METHODS:

Thirty-six male Wistar albino rats were randomly divided into four groups as follows: 1) control rats; 2) rats with experimental periodontitis (PED); 3) rats with PED treated with ALA (ALA); and 4) rats with PED treated with ALA+Vit-C (ALA+Vit-C). PED was simulated by placing ligatures around the neck of teeth for 5 weeks. After ligature removal, the PED group was given a single intragastric dose of 1 mL saline, and the ALA and ALA+Vit-C groups were treated with an intragastric dose of 50 mg/kg ALA and ALA+Vit-C for 15 days, respectively. Levels of serum bone alkaline phosphatase (B-ALP) and myeloperoxidase (MPO) activity in gingival tissues were analyzed. To evaluate the osteoclast activation, expression of activated receptor activator nuclear factor-kappa B ligand (RANKL) and bone density index (BDI) were determined stereologically in the bone sections obtained from the mandibles of the rats.

RESULTS:

The results showed statistically significant differences between the PED group and groups treated with antioxidant according to B-ALP, MPO, RANKL, and BDI values (P <0.05). ALA and ALA+Vit-C treatments showed beneficial effects on the mesial/distal periodontal bone support at the ligature-induced periodontitis tooth areas.

CONCLUSION:

This study shows that ALA and Vit-C treatment provides therapeutic effects on inhibition of alveolar bone resorption and periodontal tissue destruction.