Genotyping of Low beta-ODAP Grass Pea (Lathyrus sativus L.) Germplasm with EST-SSR Markers

Creative Commons License

ARSLAN M., BASAK M., Aksu E., UZUN B., YOL E.

BRAZILIAN ARCHIVES OF BIOLOGY AND TECHNOLOGY, cilt.63, 2020 (SCI-Expanded) identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 63
  • Basım Tarihi: 2020
  • Doi Numarası: 10.1590/1678-4324-2020190150
  • Dergi Adı: BRAZILIAN ARCHIVES OF BIOLOGY AND TECHNOLOGY
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Animal Behavior Abstracts, Aquatic Science & Fisheries Abstracts (ASFA), BIOSIS, Biotechnology Research Abstracts, CAB Abstracts, Veterinary Science Database, Directory of Open Access Journals
  • Anahtar Kelimeler: genetic diversity, microsatellite, molecular selection, SIMPLE SEQUENCE REPEAT, GENETIC DIVERSITY ANALYSIS, POPULATION-STRUCTURE, L-CICERA, POLYMORPHISM, VARIABILITY, IMPROVEMENT, CULTIVARS, PROTEIN, YIELD
  • Akdeniz Üniversitesi Adresli: Evet

Özet

Grass pea (Lathyrus sativus L.) is an important protein source in arid regions as both human and animal food. Despite its significance, the use of grass pea is limited by the presence of beta-N-oxalyl-L-a,b-diaminopropionic acid (beta-ODAP) which can cause neurological disorders. Breeding studies in grass pea have therefore focused on developing high-yielding varieties with low beta-ODAP content. However, the narrow range of genetic diversity and the restricted genomic tools in grass pea have slowed progress in such breeding. The present investigation was conducted to explore the genetic diversity of low beta-ODAP germplasm consisting of 22 accessions with 31 EST-SSR markers. The molecular analyses revealed a total of 133 alleles ranging from 142 to 330 bp with a mean number of alleles per locus of 4.29. The mean polymorphic information content (PIC) value was calculated as 0.49, and the EST-SSRs in loci S5, S6 and S116 were of the most informative PICs. A dendrogram based on Nei's genetic distance matrix revealed that breeding lines were grouped in two main clusters. Genetic distances were higher between GP6/GP11, GP4/GP11 and GP5/GP8 accessions which could be further used in crop improvement studies for developing wider genetic diversity.