Magnetic solid-phase extraction of quercetin on magnetic-activated carbon cloth (MACC)


Arain M. B., YILMAZ E., HODA N., Kazi T. G., SOYLAK M.

JOURNAL OF THE IRANIAN CHEMICAL SOCIETY, vol.16, no.7, pp.1365-1372, 2019 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Article
  • Volume: 16 Issue: 7
  • Publication Date: 2019
  • Doi Number: 10.1007/s13738-019-01622-5
  • Journal Name: JOURNAL OF THE IRANIAN CHEMICAL SOCIETY
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.1365-1372
  • Keywords: Quercetin, Magnetic solid-phase extraction, Activated carbon cloth, Spectrophotometry, ATOMIC-ABSORPTION-SPECTROMETRY, CLOUD POINT EXTRACTION, ENVIRONMENTAL-SAMPLES PRIOR, TRACE AMOUNTS, SPECTROPHOTOMETRIC DETERMINATION, FREE-RADICALS, FOOD SAMPLES, PRECONCENTRATION, SEPARATION, COPPER
  • Akdeniz University Affiliated: Yes

Abstract

Due to their small particle size and high surface to volume ratio, nanoparticles exhibit new and unique properties from their bulk counterparts. One of the promising alternative synthetic routes for metal nanoparticles is green synthesis, which employs nontoxic reactants derived from the biological sources also which make them particularly attractive for use in biomedical science. Bimetallic nanoparticles often display different or better catalytic, optical and magnetic properties, and surface energy than their monometallic counterparts due to the synergism between two metallic nanoparticles. In the present study, green synthesis of Ag, Au and Ag-Au nanoparticles was reported using the aqueous extract of turmeric powder. Phytochemicals found in the extract resulted in formation of Ag, Au and Ag-Au nanoparticles by reducing their ions to metallic form. The synthesized nanoparticles were characterized with UV-Vis spectroscopy, X-ray diffraction (XRD) and transmission electron microscopy (TEM). Also an immediate color change in the extract after addition of salts confirmed the formation of nanoparticles by reduction reactions. It was observed that SPR absorption peak for Au and Ag-Au nanoparticles at 534 and 534 nm respectively. While for Ag nanoparticles the certain absorption peak could not be observed, the obvious shoulder in the range of 430-460 nm confirmed the presence of Ag nanoparticles in the extract. The structures of nanoparticles synthesized were confirmed as crystalline form by XRD. The mean diameter of Ag, Au and bimetallic Ag-Au nanoparticles were found to be as 4±5, 9±7 and 12±4 nm respectively, by evaluating TEM images with Adobe Photoshop 7. Mostly, spherical shape was observed for the nanoparticles in TEM images. It is concluded that the confirmed method in this work is cost-effective and environmentally friendly. Thus, it can be used safely and effectively in the synthesis of Ag, Au and Ag-Au nanoparticles instead of chemical methods. 

Magnetic-activated carbon cloth (MACC) was synthesized, characterized, and used as magnetic adsorbent in magnetic solid-phase extraction of quercetin prior to UV-visible spectrophotometric determination. Different parameters such as pH, amount of MACC, effect of volume, and elution time were optimized for the determination of quercetin using UV-visible spectrophotometer at 370nm. In this procedure, quercetin molecules in aqueous sample phase were adsorbed on the 10mg of the adsorbent at pH 4.0 and desorbed with acidic methanol solution by using vortex mixer. Limit of detection (LOD), limit of quantification (LOQ), and relative standard deviation were found as 1.4ngmL(-1), 4.8ngmL(-1) and lower than 10%, respectively. The applicability of the developed magnetic solid-phase extraction method was proved on onion samples.