Gene-drive based strategy for engineering disease resistance with creating heritable mutation by using CRISPR/Cas9

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Tek M. İ., Çalış Ö.

2nd International Molecular Plant Protection Congress, Bursa, Turkey, 15 - 18 May 2023, vol.1, pp.111

  • Publication Type: Conference Paper / Summary Text
  • Volume: 1
  • City: Bursa
  • Country: Turkey
  • Page Numbers: pp.111
  • Akdeniz University Affiliated: Yes


Gene drives are a powerful new gene-editing tool that allows for the management of detrimental organisms, such as the malaria vector Anopheles gambiae, by creating heritable mutations through genetic manipulation of pest populations. The development of site-specific genome-editing tools has improved gene drives and made them a promising approach for addressing agricultural, ecological, and human health problems. This technology can be used to manage pests and weeds that reduce yield and quality in agricultural production. Additionally, there is potential to generate disease-resistant cultivars using CRISPR drives with only routine pollination after a single transformation assay, eliminating the need for repetitive gene-editing experiments or backcrossing. In this study, we designed two CRISPR/Cas9-based gene drives with constitutive or inducible promoters to disrupt the function of CsaMLO8, the major gene associated with powdery mildew (PM) susceptibility in C. sativus. We used an in silico approach to select targets for knock-in and gene-drive designing for pre-experimental guidance and identify edited plants for post-experimental analysis. Additionally, our study aims to investigate the impact of sex bias and promoter type on the efficiency of gene drives and the type and heredity of induced mutations. Our overall objective is to assess the potential of CRISPR-based gene drives as a tool for plant breeding and other methods to control harmful organisms that reduce agricultural yield or quality. By identifying the opportunities presented by CRISPR-based gene drives, we hope to provide valuable insights into effective strategies for generating disease-resistant cultivars in a shorter time and at a lower cost.