L-carnitine protects gastric mucosa by decreasing ischemia-reperfusion induced lipid peroxidation


Derin N., Izgut-Uysal V., Agac A., Aliciguzel Y., Demir N.

JOURNAL OF PHYSIOLOGY AND PHARMACOLOGY, vol.55, no.3, pp.595-606, 2004 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 55 Issue: 3
  • Publication Date: 2004
  • Journal Name: JOURNAL OF PHYSIOLOGY AND PHARMACOLOGY
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.595-606
  • Keywords: ischemia-reperfusion, lipid peroxidation, antioxidant enzymes, gastric lesion, L-carnitine, LOCAL ISCHEMIA, XANTHINE-OXIDASE, NITRIC-OXIDE, SUPEROXIDE-DISMUTASE, OXYGEN RADICALS, INDUCED LESIONS, INJURY, RATS, STRESS, MELATONIN
  • Akdeniz University Affiliated: Yes

Abstract

Studies have shown that reactive oxygen metabolites and lipid peroxidation play important roles in ischemia-reperfusion injury in many organs such as heart, brain and stomach. The aim of this study is to evaluate the antioxidant effect of L-carnitine on gastric mucosal barrier, lipid peroxidation and the activities of antioxidant enzymes in rat gastric mucosa subjected to ischemia-reperfusion injury. Rats were subjected to 30 min of ischemia followed by 60 min of reperfusion. L-carnitine (100 mg/kg), was given to rats intravenously five minutes before the ischemia. In our experiment, lesion index, thiobarbituric acid reactive substances, prostaglandin E-2 and mucus content in gastric tissue were measured. The results indicated that the lesion index and the formation of thiobarbituric acid reactive substances increased significantly with the ischemia-reperfusion injury in the gastric mucosa. L-carnitine treatment reduced these parameters to the values of sham operated rats. The tissue catalase and superoxide dismutase activities and prostaglandin E-2 production decreased significantly in the gastric mucosa of rats exposed to ischemia-reperfusion. L-carnitine pretreatment increased the tissue catalase activity and prostaglandin E-2 to the levels of sham-operated rats but did not change superoxide dismutase activity. There were no significant difference in glutathione peroxidase activity and mucus content between the groups in the gastric mucosa. In summary, L-carnitine pretreatment protected gastric mucosa from ischemia-reperfusion injury by its decreasing effect on lipid peroxidation and by preventing the decrease in prostaglandin E-2 content of gastric mucosa.