Microsatellites for genetic and taxonomic research on thyme (Thymus L.)

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KARACA M., INCE A. G., Aydin A., ELMASULU S., TURGUT K.

TURKISH JOURNAL OF BIOLOGY, cilt.39, sa.1, ss.147-159, 2015 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 39 Sayı: 1
  • Basım Tarihi: 2015
  • Doi Numarası: 10.3906/biy-1406-20
  • Dergi Adı: TURKISH JOURNAL OF BIOLOGY
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, TR DİZİN (ULAKBİM)
  • Sayfa Sayıları: ss.147-159
  • Anahtar Kelimeler: Cross-species transferable markers, genetic variations, simple sequence repeats, threatened species, EXTRACTION METHOD, LAMIACEAE, DIVERSITY, MARKERS, TISSUES, GENERA, PLANTS, FRESH, LOCI, WILD
  • Akdeniz Üniversitesi Adresli: Evet

Özet

Microsatellites are considered the marker of choice in modern research. However, there is no application of microsatellites in the research on the genus Thymus due to the scarcity of specific primer pairs. Using in silico data of several genera in the family Lamiaceae, 23 microsatellite primer pairs (LT) were developed and evaluated in 48 samples representing 9 species and subspecies of the genus Thymus. Codominant and multiallelic LT microsatellite markers were not only useful in the determination of genetic structure and variation within and between the species but also were powerful taxonomic aids in the molecular phylogeny of Thymus L. Moreover, some LT primer pairs successfully cross-amplified microsatellite markers in Salvia and Origanum, indicating that they could be used in other genera in the family Lamiaceae. To the best of our knowledge, this is the first report of genic microsatellite markers for the genus Thymus.