Synthesis and Characterization of Nickel Oxide Nanoparticles via Polyol-Mediated Hydrothermal Process with Antibacterial Properties
ChemistrySelect, vol.10, no.9, 2025 (SCI-Expanded, Scopus)
- Publication Type: Article / Article
- Volume: 10 Issue: 9
- Publication Date: 2025
- Doi Number: 10.1002/slct.202405811
- Journal Name: ChemistrySelect
- Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Chemical Abstracts Core
- Keywords: Antibacterial activity, Hydrothermal synthesis, Minimum inhibitory concentration (MIC), Nickel oxide nanoparticles (NiO NPs), PVP10K
- Akdeniz University Affiliated: Yes
Abstract
Nickel oxide nanoparticles (NiO NPs) synthesized with polyvinylpyrrolidone (PVP) demonstrate promising antimicrobial properties for biomedical applications. NiO NPs were synthesized using PVP stabilizers of varying molecular weights: PVP K10 (average MW 10,000 dalton), PVP K15 (average MW 15,000 dalton), and PVP K40 (average MW 40,000 dalton), employing a hydrothermal method. The NiO NPs synthesized with PVP10K exhibited smaller particle sizes ranging from 30 to 40 nm and a larger surface area. Optimal synthesis conditions for NiO NPs were established as follows: 100 °C for 6 h for K1-6/100, 120 °C for 6 h for K1-6/120, and 120 °C for 12 h for K1-12/120. TEM images revealed that the NiO NPs possessed a hexagonal morphology with sizes ranging between 10 and 30 nm. XRD analysis confirmed the crystalline structure of the NiO NPs, whereas FTIR spectra validated the presence of Ni─O bonds. UV–vis detected the optical bandgap within the range of 3.06 to 3.31 eV. The minimum inhibitory concentration assays indicated that the compound K1-12/120 exhibited the most pronounced antibacterial effect against Escherichia coli at a concentration of 256 µg/mL and against Staphylococcus aureus at a concentration of 128 µg/mL. Moreover, Gram-positive bacteria were found to be more susceptible to NiO NPs.