The influence of sperm/zona pellucida collision rates on zona binding
ANDROLOGIA, cilt.28, sa.5, ss.261-265, 1996 (SCI-Expanded, Scopus)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 28 Sayı: 5
- Basım Tarihi: 1996
- Doi Numarası: 10.1111/j.1439-0272.1996.tb02794.x
- Dergi Adı: ANDROLOGIA
- Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
- Sayfa Sayıları: ss.261-265
- Anahtar Kelimeler: zona pellucida binding, human sperm, collision rate, HEMIZONA ASSAY, INVITRO FERTILIZATION, HUMAN-SPERMATOZOA, INSEMINATION, INFERTILITY, CAPACITY
- Akdeniz Üniversitesi Adresli: Hayır
Özet
The satisfying success rates reported with intra-cytoplasmic sperm injection (ICSI) ureed clinicians and scientists to re-address the emphasis in the management of the male factor patient towards gamete manipulation in order to circumvent the underlying problem causing fertilization failure. We have designed a study to (i) calculate the collision rate of a sperm population with the human zona pellucida, using a mathematical hypothesis and (ii) use the calculated collision rate to evaluate subsequent zona binding results obtained under hemizona assay conditions. Microdroplets were used to co-incubate sperm and human oocytes in order to evaluate zona binding. Using microvolumes, the track followed by sperm, as well as the maximum distance travelled were employed to calculate the collision rate of sperm and zona pellucida. The sperm concentrations of swim up samples were adjusted to 4 x 10(6) and 0.8 x10(6) sperm ml(-1). Five separate droplets each of 20 mu l containing 4 x 10(6) sperm ml(-1) (80000 motile sperm) and 200 mu l containing 0.8 x 10(6) sperm ml(-1) (160000 motile sperm), respectively, were prepared. Both volumes were incubated for 18 h at 37 degrees C. The mean (+/-SD) number of spermatozoa tightly bound to hemizona, incubated in 20 mu l and 200 mu l sperm droplets, was 2444+/-612 and 548+/-315, respectively (P=0.0001). The results can be used as a guideline to calculate the optimum insemination concentration needed for a specific sperm population to ensure the maximum collision rates with the oocyte.