Akademik Veri Yönetim Sistemi
BIOTECHNOLOGY & BIOTECHNOLOGICAL EQUIPMENT, cilt.15, sa.2, ss.106-113, 2001 (SCI-Expanded)
Wild grapevine was investigated in the southwestern part of Turkey where 114 accessions were collected from forestsandbanks of the rivers. These grapes are called "Jackal grapes" by villagers. Wild grapes a small, black and nearly round berries. They had two or more seeds. Berry weight did not exceed 1g. Cluster mean weight was 33.5 g and clusters were smaller in size than those of cultivated grapes with about 40 berries per cluster. Wild grapes had a broad range of leaf shape and size. Leaf width was greater than leaf length and leaves had 3 or 5 lobes. Length of petiole was smaller than midvein. All accessions were subjected to isozymic analysis. Acid phosphatase (ACP), Catechol oxidase(CO), Glutamate oxalacetate transaminase(GOT), Malate dehydrogenase(MDH), and peroxidase(PER) enzymes were extracted from mature leaves. Electrophoresis was performed in vertical polyacrylamide slab gels (PAGE). Isoenzyme polymorphism was observed for all enzyme systems studied except GOT. This enzyme has only two isozyme bands and all accessions had the same banding pattern. PER had two zones of activity with two or three band pattern variants in each zone. Two zones were observed in Catechol oxidase (CO). The first (slower) zone had slurred bands and it was not useful for the identification of accessions. The second zone had four well resolved bands. Acid phosphotase (ACP) had two zones but there were no isozyme polymorphism in the fastest zone. The number of bands in the slower zone ranged from 1 to 3. Malate dehydrogenase (MDH) had 1-3 thin, fast migrating bands, followed by two well resolved, but slower migrating thick bands.