Akademik Veri Yönetim Sistemi
22th International Istanbul Scientific Research Congress on Life, Engineering, Architecture, and Mathematical Sciences, İstanbul, Türkiye, 20 - 22 Ağustos 2025, ss.421-422, (Özet Bildiri)
Rapid advances in genomics have made high-throughput genetic data widely used across biology. Pioneering biodiversity studies have applied these data to investigate evolutionary patterns, phylogenetic relationships, and phylogeography. In particular, mitochondrial genomes (mitogenomes) have been adopted as primary markers in many animal groups because they exhibit limited DNA repair mechanisms, little or no recombination, rapid evolution, and matrilineal inheritance. Despite the rising popularity of mitogenomes over the last decade, representation across taxa remains limited in most animal groups. Orthoptera, one of the most species-rich insect orders, comprises more than 30,459 valid species (Cigliano et al. 2025), yet complete mitogenomes are available for only ~334 species (≈1.1%)(NCBI, https://www.ncbi. nlm.nih.gov/genbank/). This limited representation underscores the need for additional mitogenomic data from Orthoptera to achieve a more comprehensive understanding of their evolutionary history.
Here, the complete mitogenome of the Anatolian endemic katydid Uvarovistia munzurensis (Tettigoniidae: Pholidopterini) is reported for the first time. The mitogenome was assembled using Getorganelle v. 1.7. (Jin et al. 2020) with animal mitogenome dataset and annotated via MITOS2 webserver (Donath et al. 2019), with tRNA secondary structures verified during annotation. The circular genome is 15,914 bp in length and comprises 13 protein-coding genes (PCGs), 22 tRNAs, 2 rRNAs, and a control (AT-rich) region. Gene content and strand orientation, gene lengths, nucleotide composition, AT-skew [(A−T)/(A+T)] per gene, codon-position composition for PCGs, start and stop codons of PCGs, tRNA anticodons, amino acid composition, and relative synonymous codon usage per PCG were characterized. Start codons showed the typical ATN (except CAA in cox1), with complete or incomplete stop codons (T--/TA-) and the amino acid composition of the protein-coding genes was biased toward leucine, serine, and phenylalanine, consistent with patterns observed in Orthoptera and other insect mitogenomes (Öztürk & Çıplak, 2019; Huang et al. 2023; Guo et al. 2023). Codon usage and AT-skew analyses revealed a strong bias toward A/U-ending codons, consistent with the genome’s high AT-rich nature and likely driven by mutational pressure and selection (Hershberg &Petrov, 2008; Guan et al. 2019; Wang et al. 2018). Overall, the features indicate close conformity to the ancestral pancrustacean/insect mitogenome (Cameron 2014; Sheffield et al. 2008; Song et al. 2015). This newly generated mitogenome will provide an important reference for future phylogenetic and phylogeographic studies within Pholidopterini and will contribute conservation genetics efforts for the largely endemic Orthoptera fauna of Anatolia.
Keywords: Mitogenome characterization, Orthoptera, Uvarovistia