Irreversible and reversible pore formation by polymeric alkylpyridinium salts (poly-APS) from the sponge Reniera sarai
BRITISH JOURNAL OF PHARMACOLOGY, cilt.139, sa.8, ss.1399-1408, 2003 (SCI-Expanded, Scopus)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 139 Sayı: 8
- Basım Tarihi: 2003
- Doi Numarası: 10.1038/sj.bjp.0705374
- Dergi Adı: BRITISH JOURNAL OF PHARMACOLOGY
- Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
- Sayfa Sayıları: ss.1399-1408
- Anahtar Kelimeler: pore former, sponge toxin, polymeric alkylpyridinium salt, halitoxins, intracellular calcium, sensory neurone, CALCIUM-CHANNEL CURRENTS, 3-ALKYLPYRIDINIUM POLYMERS, AMPHIMEDON VIRIDIS, HALITOXIN, CELLS
- Açık Arşiv Koleksiyonu: AVESİS Açık Erişim Koleksiyonu
- Akdeniz Üniversitesi Adresli: Hayır
Özet
1 In this study, we investigated the electrophysiological actions of a high molecular weight fraction, predominantly containing two polymeric 1,3-alkylpyridinium salts (poly-APS) of 5.5 and similar to19 kDa isolated from the marine sponge Reniera sarai. The biological properties of poly-APS are of particular interest because this preparation may be used to deliver macromolecules into the intracellular environment without producing long-term damage to cells. Poly-APS (50-0.05 mug ml(-1)) was applied to cultured dorsal root ganglion neurones or HEK 293 cells and changes in cell membrane properties were measured using whole-cell patch-clamp recording and fura-2 Ca2+ imaging.