Irreversible and reversible pore formation by polymeric alkylpyridinium salts (poly-APS) from the sponge Reniera sarai

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McClelland D., Evans R., Abidin I., Sharma S., Choudhry F., Jaspars M., ...More

BRITISH JOURNAL OF PHARMACOLOGY, vol.139, no.8, pp.1399-1408, 2003 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 139 Issue: 8
  • Publication Date: 2003
  • Doi Number: 10.1038/sj.bjp.0705374
  • Journal Name: BRITISH JOURNAL OF PHARMACOLOGY
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.1399-1408
  • Keywords: pore former, sponge toxin, polymeric alkylpyridinium salt, halitoxins, intracellular calcium, sensory neurone, CALCIUM-CHANNEL CURRENTS, 3-ALKYLPYRIDINIUM POLYMERS, AMPHIMEDON VIRIDIS, HALITOXIN, CELLS
  • Akdeniz University Affiliated: No

Abstract

1 In this study, we investigated the electrophysiological actions of a high molecular weight fraction, predominantly containing two polymeric 1,3-alkylpyridinium salts (poly-APS) of 5.5 and similar to19 kDa isolated from the marine sponge Reniera sarai. The biological properties of poly-APS are of particular interest because this preparation may be used to deliver macromolecules into the intracellular environment without producing long-term damage to cells. Poly-APS (50-0.05 mug ml(-1)) was applied to cultured dorsal root ganglion neurones or HEK 293 cells and changes in cell membrane properties were measured using whole-cell patch-clamp recording and fura-2 Ca2+ imaging.