Genetic diversity and population structure in Turkish barley (Hordeum vulgare L.) cultivars using start codon targeted (SCoT) markers


Yeken M. Z., Emiralioğlu O., TEKİN M., Çelik A., Çiftçi V., Özer G.

Genetic Resources and Crop Evolution, 2024 (SCI-Expanded) identifier

  • Yayın Türü: Makale / Tam Makale
  • Basım Tarihi: 2024
  • Doi Numarası: 10.1007/s10722-024-02297-5
  • Dergi Adı: Genetic Resources and Crop Evolution
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Agricultural & Environmental Science Database, BIOSIS, Biotechnology Research Abstracts, CAB Abstracts, Geobase, Veterinary Science Database
  • Anahtar Kelimeler: Barley, Genetic diversity, Hordeum vulgare, Pre-breeding, Variety
  • Akdeniz Üniversitesi Adresli: Evet

Özet

Barley is a significant cereal crop, extensively used for food, animal feed, and as a fundamental raw material in the production of various beverages. Türkiye holds a prominent position as one of the global centers of diversity for cereal crops, including barley. However, the genetic diversity among barley cultivars registered in Türkiye has not yet been comprehensively investigated. In this study, the genetic diversity of 92 traditional and modern barley cultivars, registered between 1963 and 2019, was investigated using start codon targeted (SCoT) markers. A total of 125 bands were identified, 95 of which exhibited polymorphism, resulting in a polymorphic ratio of 76.0%. The proportion of polymorphic bands ranged from 58.82 to 85.71%, with an average of 76.26%. Furthermore, no significant genetic diversity differences were observed between two-row and six-row barley types. Analysis of molecular variance revealed that the majority of genetic variation occurred within populations (90%), while variation among populations accounted for 10%. Additional genetic diversity analyses, including principal coordinate analysis, neighbor-joining clustering and bayesian model-based clustering, were conducted. The population structure analysis distinctly identified two hypothetical clusters, with proportions of 41.3% and 58.7%, respectively. Overall, a substantial level of genetic diversity was detected among the cultivars; however, it was evident that traditional and modern barley cultivars were grouped together, indicating no significant genetic distinction between them concerning the SCoT markers analyzed. These findings provide valuable insights for barley breeding programs.