Ameliorative effects of melatonin against nano and ionic cobalt induced genotoxicity in twoin vivoDrosophila assays

ERTUĞRUL H., YALÇIN B., GÜNEŞ M., KAYA B.

DRUG AND CHEMICAL TOXICOLOGY, cilt.43, sa.3, ss.279-286, 2020 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 43 Sayı: 3
  • Basım Tarihi: 2020
  • Doi Numarası: 10.1080/01480545.2019.1585444
  • Dergi Adı: DRUG AND CHEMICAL TOXICOLOGY
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, BIOSIS, CAB Abstracts, EMBASE, Environment Index, Food Science & Technology Abstracts, International Pharmaceutical Abstracts, MEDLINE, Veterinary Science Database
  • Sayfa Sayıları: ss.279-286
  • Anahtar Kelimeler: Nanoparticle, cobalt, Drosophila, single cell gel electrophoresis assay, somatic mutation and recombination test, melatonin, WING-SPOT-TEST, COPPER-OXIDE NANOPARTICLES, HYPOXIA-INDUCIBLE FACTOR, DROSOPHILA-MELANOGASTER, OXIDATIVE STRESS, OCCUPATIONAL-EXPOSURE, SILVER NANOPARTICLES, BENZYL DERIVATIVES, ASCORBIC-ACID, COMET ASSAY
  • Akdeniz Üniversitesi Adresli: Evet

Özet

The aim of this study is to evaluate the ameliorative effect of melatonin (MEL) against induced genotoxicity by cobalt (II) chloride (CoCl2) and cobalt nanoparticles (CoNPs) (50 nm). Genotoxicity of CoCl(2)and CoNPs were investigated using single cell gel electrophoresis (COMET) inDrosophila melanogasterhemocytes, which are blood cells of theDrosophila,and the somatic mutation and recombination test (SMART) was used to investigate mutant effects on theDrosophilawings. Three concentrations (0.1, 1, and 10 mM) of CoNPs and CoCl(2)were applied to demonstrate their genotoxic potential. Both CoNPs and CoCl(2)have mutagenic potential for the three concentrations tested in the COMET assay; however, only the 10 mM concentration of the ionic form and two high concentrations (1 and 10 mM) of CoNPs induced genotoxicity in theDrosophilaSMART assay. Three different concentrations of MEL (0.1, 0.5 and 2.5 mM) were used against cobalt at highest concentration (10 mM) of both CoCl(2)and CoNPs in both the SMART and COMET assays. MEL ameliorated the genotoxicity induced by CoCl(2)and CoNPsin vivo DrosophilaCOMET and SMART assays.