AZF microdeletions on the Y chromosome of infertile men from Turkey

Sargin C., Berker-Karauzum S., Manguoglu E., Erdogru T., Karaveli S., Gulkesen K. H., ...Daha Fazla

ANNALES DE GENETIQUE, cilt.47, sa.1, ss.61-68, 2004 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 47 Sayı: 1
  • Basım Tarihi: 2004
  • Doi Numarası: 10.1016/j.anngen.2003.09.002
  • Dergi Adı: ANNALES DE GENETIQUE
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.61-68
  • Anahtar Kelimeler: infertility, azoospermia, oligozoospermia, microdeletion, SEVERE OLIGOZOOSPERMIA, AZOOSPERMIA, DELETIONS, SPERMATOGENESIS, GENE, SUBREGIONS, CELLS
  • Akdeniz Üniversitesi Adresli: Evet

Özet

Intervals V and VI of Yq11.23 regions contain responsible genes for spermatogenesis, and are named as "azoospermia factor locus" (AZF). Deletions in these genes are thought to be pathogenetically involved in some cases of male infertility associated with azoospermia or oligozoospermia. The aim of this study was to establish the prevalence of microdeletions on the Y chromosome in infertile Turkish males with azoospermia or oligozoospermia. We applied multiplex polymerase chain reaction (PCR) using several sequence-tagged site (STS) primer sets, in order to determine Y chromosome microdeletions. In this study, 61 infertile males were enrolled for the molecular AZF screening program. In this cohort, one infertile male had 46,XX karyotype and the remaining had 46,XY karyotypes. Forty-eight patients had a diagnosis of azoospermia and 13 had oligozoospermia. Microdeletions in AZFa, AZFb and AZFc (DAZ gene) regions were detected in two of the 60 (3.3%) idiopathic infertile males with normal karyotypes and a SRY translocation was determined on 46,XX male. Our findings suggest that genetic screening should be advised to infertile men before starting assisted reproductive treatments. (C) 2003 Elsevier SAS. All rights reserved.