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EUROPEAN JOURNAL OF PHARMACOLOGY, vol.437, no.1-2, pp.97-103, 2002 (SCI-Expanded)
We investigated whether the glutamergic system plays a role in isolated trachea from control and ovalbumin-sensitized guinea-pigs. Electrical field stimulation induced contractile responses in control group, but electrical field stimulation produced relaxation responses in ovalbumm-challenged guinea-pigs. The responses induced by, electrical field stimulation in both groups were completely abolished by tetrodotoxin, but unaffected by hexamethonium. DL2-amino-5-phosphono-valeric acid (D-AP5) caused a concentration-dependent statistically significant inhibition in the contractile responses to electrical field stimulation(50) (EFS50) in control guinea-pigs. But in the ovalbumin-challenged groups, D-AP5 did not cause any significant effect on the relaxation response to frequency of field stimulation (EFS50). N-G-montnethyl-L-argine caused a significant inhibition in the relaxation effect of EFS50. L- and D-glutamate and N-methyl-D-aspartic acid (NMDA) alone had no effect on the resting tension on the trachea in both groups. Carbachol produced concentration-dependent contractile responses in ovalbumin-challenged groups. These results suggested that responses to electrical field stimulation in control groups might be due to NMDA receptor-mediated release of any substance on prejunctional neurotics and, alternatively, NMDA might exert a modulatory effect on any substance at prejuntional level. Also, responses to electrical field stimulation in ovalbumin-challenged guinea-pigs might not be mediated by NMDA but rather by increasing the production of nitric oxide by inducible nitric oxide synthase. (C) 2002 Elsevier Science B.V All rights reserved.