Role of TNF-related apoptosis-inducing ligand (TRAIL) in the pathogenesis of varicocele-induced testicular dysfunction

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Celik O., Kutlu O., Tekcan M., Celik-Ozenci C., KÖKSAL İ. T.

ASIAN JOURNAL OF ANDROLOGY, cilt.15, sa.2, ss.269-274, 2013 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 15 Sayı: 2
  • Basım Tarihi: 2013
  • Doi Numarası: 10.1038/aja.2012.112
  • Dergi Adı: ASIAN JOURNAL OF ANDROLOGY
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.269-274
  • Anahtar Kelimeler: apoptosis, infertility, testis, TNF-related apoptosis inducing ligand (TRAIL), varicocele, NF-KAPPA-B, DECOY RECEPTOR, EXPRESSION, IDENTIFICATION, ACTIVATION, PROTECTS, THERAPY
  • Akdeniz Üniversitesi Adresli: Evet

Özet

The higher frequency of varicocele in men with infertility has drawn attention and resulted in increased research at the molecular level towards treatments. The aim of this study was to investigate the role of tumor necrosis factor (TNF)-related apoptosis-inducing ligand (TRAIL) and its receptors in varicocele-induced testicular dysfunction in an experimental rat model. The rats were divided into three groups: control, sham and varicocele. Varicoceles in rats were induced by partial ligation of the left renal vein and left testes. The rats were analyzed 13 weeks after surgery. The degree of DNA fragmentation within cells in the testis was determined using terminal deoxynucleotidyl transferase deoxyuridine triphosphate nick end labeling (TUNEL) assay. Tubule degeneration was evaluated using the Johnsen score. The expression of TRAIL and its receptors was detected by immunohistochemical and Western blotting techniques. The apoptotic index, Johnsen score and the expression of TRAIL and TRAIL receptors were examined. The data are presented as the mean+/-s.d. and were analyzed using computer software. The Kruskal-Wallis and Dunn's multiple comparison tests were used in the statistical analyses. The germ cell apoptotic index was increased in rats with varicoceles when compared with the sham and control groups (P=0.0031). The Johnsen score was significantly decreased in the varicocele group when compared with the sham and control groups (P<0.0001). Immunohistochemical and Western blotting analyses showed that after varicocele induction, the expression of TRAIL-R1 and TRAIL-R4 in germ cells was increased and the expression of TRAIL-R2 was decreased. There are no significant differences among the groups in terms of TRAIL and TRAIL-R3 receptor expression. The results of this study indicate that TRAIL and its receptors may have a potential role in the pathogenesis of varicocele-induced testicular dysfunction.

Role of TNF-related apoptosis-inducing ligand (TRAIL) in the pathogenesis of varicocele-induced testicular dysfunction

By:Celik, O (Celik, Orcun)[ 1 ] ; Kutlu, O (Kutlu, Omer)[ 2 ] ; Tekcan, M (Tekcan, Merih)[ 3 ] ; Celik-Ozenci, C (Celik-Ozenci, Ciler)[ 3 ] ; Koksal, IT (Koksal, Ismail T.)[ 1 ]

 

ASIAN JOURNAL OF ANDROLOGY

 

Volume: 15

Issue: 2

Pages: 269-274

Special Issue: SI

DOI: 10.1038/aja.2012.112

Published: MAR 2013

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ASIAN JOURNAL OF ANDROLOGY Open access indicator

JCR® Category Quartile in Category
ANDROLOGY Q2
UROLOGY & NEPHROLOGY Q2

Data from the 2012 edition of Journal Citation Reports®

Publisher ACTA PHARMACOLOGICA SINICA, 294 TAI-YUAN RD, SHANGHAI, 200031, PEOPLES R CHINA

ISSN: 1008-682X

Research Domain Endocrinology & Metabolism
Urology & Nephrology

Abstract

The higher frequency of varicocele in men with infertility has drawn attention and resulted in increased research at the molecular level towards treatments. The aim of this study was to investigate the role of tumor necrosis factor (TNF)-related apoptosis-inducing ligand (TRAIL) and its receptors in varicocele-induced testicular dysfunction in an experimental rat model. The rats were divided into three groups: control, sham and varicocele. Varicoceles in rats were induced by partial ligation of the left renal vein and left testes. The rats were analyzed 13 weeks after surgery. The degree of DNA fragmentation within cells in the testis was determined using terminal deoxynucleotidyl transferase deoxyuridine triphosphate nick end labeling (TUNEL) assay. Tubule degeneration was evaluated using the Johnsen score. The expression of TRAIL and its receptors was detected by immunohistochemical and Western blotting techniques. The apoptotic index, Johnsen score and the expression of TRAIL and TRAIL receptors were examined. The data are presented as the mean+/-s.d. and were analyzed using computer software. The Kruskal-Wallis and Dunn's multiple comparison tests were used in the statistical analyses. The germ cell apoptotic index was increased in rats with varicoceles when compared with the sham and control groups (P=0.0031). The Johnsen score was significantly decreased in the varicocele group when compared with the sham and control groups (P<0.0001). Immunohistochemical and Western blotting analyses showed that after varicocele induction, the expression of TRAIL-R1 and TRAIL-R4 in germ cells was increased and the expression of TRAIL-R2 was decreased. There are no significant differences among the groups in terms of TRAIL and TRAIL-R3 receptor expression. The results of this study indicate that TRAIL and its receptors may have a potential role in the pathogenesis of varicocele-induced testicular dysfunction.