Akademik Veri Yönetim Sistemi
12. International Summit Scientific Research Congress, Gaziantep, Türkiye, 29 - 31 Mayıs 2024, ss.151-155
Myostatin (MSTN) is one of the promising genes to improve meat production due to playing a key role in skeletal muscle development. Mutations in the MSTN gene, which could be detected via different molecular genotyping methods, are the potential to cause double muscling phenotype and to increase meat yield in cattle populations. In this study, nt821(del11) polymorphism of the MSTN gene, defining 11 base pair deletion between 821 and 831 nucleotide positions, were investigated in two native Turkish cattle breeds known as Anatolian Black (AB) and East Anatolian Red (EAR). DNA was extracted from blood samples collected from a total of 72 animals via a salting-out isolation method. The success of the DNA extraction stage was confirmed by 1% agarose gel electrophoresis. Allele-specific polymerase chain reaction (AS-PCR) method was preferred to genotype each animal in terms of the MSTN gene. Via this molecular genotyping method, both wild and mutant alleles were expected to amplify in PCR stage by using a common forward and two reverse primers. 3% agarose gel electrophoresis was utilized to visualize wild and mutant alleles per each animal. In this study, the AS-PCR method showed that no genetic variation was present in two Anatolian cattle breeds in which all animals were detected to carry wild alleles. Due to a lack of genetic variation, MSTN was found to be inefficient in improving meat production in native Turkish cattle breeds. However, it is known that exotic cattle breeds are introduced into the country due to artificial insemination practices. Depending on the used sperms of the exotic breeds, causative mutations in the MSTN gene may be observed in cattle populations raised in Türkiye in the future. It is noteworthy that mutations in the MSTN gene may not only increase meat yield but also it may cause calving difficulties due to extending the gestation period. Therefore, native Turkish cattle breeds should be periodically screened in terms of the MSTN genes, whereas further studies may focus on other meat production-related genes to design marker-assisted selection (MAS) studies.