Simple sequence repeats as useful resources to study transcribed genes of cotton

SAHA S., Karaca M., JENKINS J., ZIPF A., REDDY O., KANTETY R.

EUPHYTICA, cilt.130, sa.3, ss.355-364, 2003 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 130 Sayı: 3
  • Basım Tarihi: 2003
  • Doi Numarası: 10.1023/a:1023077209170
  • Dergi Adı: EUPHYTICA
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.355-364
  • Anahtar Kelimeler: cotton, differential display, EST, SSR, DIFFERENTIAL DISPLAY, MESSENGER-RNA, MICROSATELLITE, ABUNDANCE
  • Akdeniz Üniversitesi Adresli: Evet

Özet

Microsatellites or Simple Sequence Repeats (SSRs) are informative molecular genetic markers in many crop species. SSRs are PCR-based, highly polymorphic, abundant, widely distributed throughout the genome and inherited in a co-dominant manner in most cases. Here we describe the presence of SSRs in cDNAs of cotton. Thirty one SSR primer pairs of 220 (similar to14%) tested led to PCR amplification of discrete fragments using cotton leaf cDNA as template. Sequence analysis showed 25% of 24 randomly selected cDNA clones amplified with different SSR primer pairs contained repeat motifs. We further showed that sequences from the SSR-containing cDNAs were conserved across G. barbadense and G. hirsutum, revealing the importance of the SSR markers for comparative mapping of transcribed genes. Data mining for plant SSR-ESTs from the publicly available databases identified SSRs motifs in many plant species, including cotton, in a range of 1.1 to 4.8% of the submitted ESTs for a given species.