A rapid detection of multidrug-resistant Mycobacterium tuberculosis by a nitrate reductase assay on blood agar

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Coban A. Y., Cayci Y. T., Deveci A., AKGUNES A., UZUN M., Durupinar B.

MEMORIAS DO INSTITUTO OSWALDO CRUZ, cilt.106, sa.3, ss.378-380, 2011 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 106 Sayı: 3
  • Basım Tarihi: 2011
  • Doi Numarası: 10.1590/s0074-02762011000300022
  • Dergi Adı: MEMORIAS DO INSTITUTO OSWALDO CRUZ
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.378-380
  • Anahtar Kelimeler: Mycobacterium tuberculosis, nitrate reductase assay, blood agar, COLORIMETRIC METHOD, LIQUID CULTURES, SPUTUM SAMPLES, SUSCEPTIBILITY, RESAZURIN, RIFAMPICIN, DRUGS
  • Akdeniz Üniversitesi Adresli: Hayır

Özet

The susceptibility of 49 Mycobacterium tuberculosis clinical isolates to isoniazid (INH) and rifampisin (RIF) (28 multi-drug resistant-tuberculosis samples) was determined by a nitrate reductase assay (NRA) on blood agar. Agreement between the NRA and other testing methods was found to be 93.8% for both INH and RIF. The sensitivity, specificity, positive predictive value and negative predictive value for INH were 92.8%, 94.2%, 86.6% and 97%, respectively. The sensitivity, specificity, positive predictive value and negative predictive value for RIF were 90.4%, 96.4%, 95% and 93.1%. In conclusion, we show here that blood agar can be used effectively for the NRA test.