A rapid detection of multidrug-resistant Mycobacterium tuberculosis by a nitrate reductase assay on blood agar


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Coban A. Y., Cayci Y. T., Deveci A., AKGUNES A., UZUN M., Durupinar B.

MEMORIAS DO INSTITUTO OSWALDO CRUZ, vol.106, no.3, pp.378-380, 2011 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 106 Issue: 3
  • Publication Date: 2011
  • Doi Number: 10.1590/s0074-02762011000300022
  • Journal Name: MEMORIAS DO INSTITUTO OSWALDO CRUZ
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.378-380
  • Keywords: Mycobacterium tuberculosis, nitrate reductase assay, blood agar, COLORIMETRIC METHOD, LIQUID CULTURES, SPUTUM SAMPLES, SUSCEPTIBILITY, RESAZURIN, RIFAMPICIN, DRUGS
  • Akdeniz University Affiliated: No

Abstract

The susceptibility of 49 Mycobacterium tuberculosis clinical isolates to isoniazid (INH) and rifampisin (RIF) (28 multi-drug resistant-tuberculosis samples) was determined by a nitrate reductase assay (NRA) on blood agar. Agreement between the NRA and other testing methods was found to be 93.8% for both INH and RIF. The sensitivity, specificity, positive predictive value and negative predictive value for INH were 92.8%, 94.2%, 86.6% and 97%, respectively. The sensitivity, specificity, positive predictive value and negative predictive value for RIF were 90.4%, 96.4%, 95% and 93.1%. In conclusion, we show here that blood agar can be used effectively for the NRA test.