Green algae Chlamydomonas reinhardtii possess endogenous sialylated N-glycans

Mamedov T., Yusibov V.

FEBS OPEN BIO, vol.1, pp.15-22, 2011 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 1
  • Publication Date: 2011
  • Doi Number: 10.1016/j.fob.2011.10.003
  • Journal Name: FEBS OPEN BIO
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.15-22
  • Keywords: Chlamydomonas reinhardtii, Mass spectrometry analysis, N-Glycan structure, Sialic acid
  • Akdeniz University Affiliated: No

Abstract

Green algae have a great potential as biofactories for the production of proteins. Chlamydomonas reinhardtii, a representative of eukaryotic microalgae, has been extensively used as a model organism to study light-induced gene expression, chloroplast biogenesis, photosynthesis, light perception, cell-cell recognition, and cell cycle control. However, little is known about the glycosylation machinery and N-linked glycan structures of green algae. In this study, we performed mass spectrometry analysis of N-linked oligosaccharides released from total extracts of Chlamydomonas reinhardtii and demonstrated that C. reinhardtii algae possess glycoproteins with mammalian-like sialylated N-linked oligosaccharides. These findings suggest that C. reinhardtii may be an attractive system for expression of target proteins. (C) 2011 Federation of European Biochemical Societies. Published by Elsevier B. V. All rights reserved.