Akademik Veri Yönetim Sistemi
International Congress on Agriculture in Alanya, Agriculture in the World (ICAAW2021), Antalya, Türkiye, 29 - 31 Ekim 2021, ss.181-189
The study was planned after the ring spot symptoms of Tomato spotted wilt virus (TSWV), which is characterized in pepper plants, and also seen in eggplant plants grown in Antalya. In this context, a total of 101 eggplant samples were collected, of which 32 from Alanya, 33 from Serik, and 36 from Kumluca. The symptoms observed in the leaves and fruits of the eggplant plant were associated with the typical ring spot symptom of TSWV in tomato and pepper fruits, and the presence of the virus in the eggplant samples was determined by RT-PCR method. Coat protein-specific primers are incapable of detecting any mutations in the TSWV genome. In this study, a primer pair was designed that enables the identification of TSWV-RB isolate in eggplants infected with TSWV and covers the C118Y mutation region in the genome of the virus. Sequence analysis of the RT-PCR product was performed with primers that amplify the mutation region and because of contig studies with Non-resistance isolates (NRB) that did not break resistance, it was revealed that eggplant plants were infected with TSWV-RB isolate, contained nucleotide change in the C118Y mutation region, and that TSWV-RB isolate was also the host. The primers that identified the TSWV-RB isolate and developed in this study give successful results in qRT-PCR studies and Melt Curve analyzes. Thanks to these primers, it has been possible to detect easily and quickly that the infection in many different hosts such as tomatoes, peppers, eggplants infected with TSWV is caused by the isolate that breaks the resistance.
Keywords: Eggplant, TSWV, Resistance-breaking, RT-PCR, qRT-PCR