Comparison of Four Methods to extract Bacterial DNA from Infected Fish Tissue

Tulay C. I.

RESEARCH JOURNAL OF BIOTECHNOLOGY, vol.8, no.10, pp.52-55, 2013 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Article
  • Volume: 8 Issue: 10
  • Publication Date: 2013
  • Journal Name: RESEARCH JOURNAL OF BIOTECHNOLOGY
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.52-55
  • Keywords: FTA card, Renibacterium salmonarium, 16S rDNA, RENIBACTERIUM-SALMONINARUM, KIDNEY-DISEASE, PCR, SAMPLES, PAPER
  • Akdeniz University Affiliated: Yes

Abstract

This study is concerned with single-step bacterial DNA extraction of Renibacterium salmonarium from infected Rainbow Trout (Oncorhynchus mykiss) kidney tissue. We examined and compared four commonly used DNA extraction protocols to determine the reliable and practical method from tissue without isolating bacteria. A total of sixty extracted DNA samples from fish tissues have been tested to show their ability to amplification of R. salmonarium 16S rDNA gene region. Results of our comparison showed that the extraction of bacterial DNA from FTA card technology is more simple, reproducible and inexpensive.