Atıf İçin Kopyala
Sahiner I. F., Soylu H., Ates E., Acar N., Üstünel İ., Danışman A.
INTERNATIONAL BRAZ J UROL, cilt.44, sa.5, ss.1012-1020, 2018 (SCI-Expanded)
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Yayın Türü:
Makale / Tam Makale
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Cilt numarası:
44
Sayı:
5
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Basım Tarihi:
2018
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Doi Numarası:
10.1590/s1677-5538.ibju.2017.0713
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Dergi Adı:
INTERNATIONAL BRAZ J UROL
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Derginin Tarandığı İndeksler:
Science Citation Index Expanded (SCI-EXPANDED), Scopus
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Sayfa Sayıları:
ss.1012-1020
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Anahtar Kelimeler:
Interstitial cystitis, Inflammation, Hyaluronic Acid, SYNDROME/INTERSTITIAL CYSTITIS, PROTAMINE SULFATE, THERAPY, PAIN, INTERLEUKIN-6, POTASSIUM, WOMEN
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Akdeniz Üniversitesi Adresli:
Evet
Özet
Objective: To evaluate the effect of intravesical hyaluronic acid (HA) treatment on inflammatory cells and the severity of inflammation in an interstitial cystitis rat model created with hydrogen chloride (HCL) via immunohistochemical studies and myeloperoxidase activity for the first time in the literature.
Materials and Methods: A total of 30 adult female white Rattus Norvegicus rats were divided into 3 groups as the HCL group, hyaluronic acid treatment (HCL-HA) group and control group. Chemical cystitis was created by administering HCL(400 microL,10 mM) except control group. A single dose of intravesical HA(0.5 mL,0.8 mg/mL) was administered to the treatment group. The bladder tissues of all subjects were immunohistochemically stained. The cell surface markers were used to evaluate inflammatory cell infiltration. Mast cell activation and IL-6 was evaluated to assess the inflammation
and severity of inflammation, respectively. Myeloperoxidase activity was measured as it shows neutrophil density. Statistical significance was accepted as P<0.05.
Results: It was observed that there was rich monocyte, T lymphocyte, B lymphocyte, and Natural Killer cells infiltration and high IL-6 levels in the bladder tissue after the intravesical hydrogen chloride instillation, especially in the stroma layer(p<0.005). In the HCL-HA group, severity of inflammation had statistically significantly regressed to the levels of the control group(p<0.005). An increase was observed in the bladder myeloperoxidase activity of the HCL group compared to the other two groups(p<0.05).
Conclusions: Single dose intravesical hyluronic acid instillation reduces inflammatory cell infiltration and the severity of bladder inflammation in the rat model of bladder pain syndrome/interstitial cystitis.