Alteration of drug metabolizing enzymes in sulphite oxidase deficiency

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Tutuncu B., Kucukatay V., Arslan S., Sahin B., Semiz A., ŞEN A.

JOURNAL OF CLINICAL BIOCHEMISTRY AND NUTRITION, cilt.51, sa.1, ss.50-54, 2012 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 51 Sayı: 1
  • Basım Tarihi: 2012
  • Doi Numarası: 10.3164/jcbn.11-79
  • Dergi Adı: JOURNAL OF CLINICAL BIOCHEMISTRY AND NUTRITION
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.50-54
  • Anahtar Kelimeler: sulphite oxidase deficiency, sulphite sensitivity, drug metabolizing enzymes, cytochrome P450s, rat, GLUTATHIONE S-TRANSFERASES, MULLET LIZA-SALIENS, LIVER-MICROSOMES, SULFUR-DIOXIDE, SENSITIVITY, EXPRESSION, RATS, PURIFICATION, DEALKYLATION, HIPPOCAMPUS
  • Akdeniz Üniversitesi Adresli: Hayır

Özet

The aim of this study was to investigate the possible effects of sulphite oxidase (SOX, E.C. 1.8.3.1) deficiency on xenobiotic metabolism. For this purpose, SOX deficiency was produced in rats by the administration of a low molybdenum diet with concurrent addition of 200 ppm tungsten to their drinking water. First, hepatic SOX activity in deficient groups was measured to confirm SOX deficiency. Then, aminopyrine N-demethylase, aniline 4-hydroxylase, aromatase, caffeine N-demethylase, cytochrome b5 reductase, erythromycin N-demethylase, ethoxyresorufin O-deethylase, glutathione S-transferase, N-nitrosodimethylamine N-demethylase and penthoxyresorufin O-deethylase activities were determined to follow changes in the activity of drug metabolizing enzymes in SOX-deficient rats. Our results clearly demonstrated that SOX deficiency significantly elevated A4H, caffeine N-demethylase, erythromycin N-demethylase and N-nitrosodimethylamine N-demethylase activities while decreasing ethoxyresorufin O-deethylase and aromatase activities. These alterations in drug metabolizing enzymes can contribute to the varying susceptibility and response of sulphite-sensitive individuals to different drugs and/or therapeutics used for treatments.