Polymorphic Microsatellite Markers Transferable Across Capsicum Species

İNCE A. G., KARACA M., ONUS A. N.

PLANT MOLECULAR BIOLOGY REPORTER, cilt.28, sa.2, ss.285-291, 2010 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 28 Sayı: 2
  • Basım Tarihi: 2010
  • Doi Numarası: 10.1007/s11105-009-0151-y
  • Dergi Adı: PLANT MOLECULAR BIOLOGY REPORTER
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.285-291
  • Anahtar Kelimeler: EST-microsatellites, Cross-amplification, Touch-down PCR, SIMPLE SEQUENCE REPEATS, SSR-MARKERS, DNA-SEQUENCE, EST-SSRS, EVOLUTIONARY, PROGRAM, NETWORK, GENES, END, MAP
  • Akdeniz Üniversitesi Adresli: Evet

Özet

Pepper (Capsicum annuum L.) is one of the most important crops in the family Solanaceae. However, the number of polymorphic molecular loci detected in this important crop is far behind that of other cultivated plant species. In the present study, a total of 45 microsatellite primer pairs were developed using Capsicum expressed sequence tags databases. Microsatellite primer pairs were tested using several species of Capsicum and several genera in the family Solanaceae including tomato, potato, eggplant, and tobacco. Results indicated that microsatellite primer pairs amplified genomic targets of C. annuum L., Capsicum baccatum L., Capsicum chacoense L., Capsicum chinense L., Capsicum frutescens L., and Capsicum pubescens Ruiz et Pavon, indicating species transferability within Capsicum. Further analyses revealed that amplicons of these primer pairs segregated 1:2:1 or 3:1 Mendelian fashions in 38 F-2 individuals of pepper. It was also noted that markers derived from sequences containing dinucleotide repeats were generally more polymorphic at the intraspecific level than sequences containing trinucleotide repeats. All the microsatellite primer pairs developed in this study will be useful for marker-assisted selection and mapping studies in pepper.