VII. INTERNATIONAL AGRICULTURAL, BIOLOGICAL AND LIFE SCIENCE CONFERENCE (AGBIOL), İstanbul, Türkiye, 7 - 10 Eylül 2025, cilt.1, ss.86, (Özet Bildiri)
Turkish Kırkağaç type melon is one of most
grown melon in Turkiye because of its well-known taste. The Kırkağaç melon
cultivars have several threats during their production including soil borne
fungal diseases. Fusarium
oxysporum fsp. melonis (FOM)
is a soil borne fungal pathogen causing serious wilting, necrosis on plant
leaves than plants deaths occurring. The aims of this study is to isolate the
Fusarium wilt pathogens from infected Kırkağaç melon plants. For the isolation
of Fusarium pathogens, infected Kırkağaç melon plants are
collected either field or greenhouse growing areas with guided-statistical
sampling method. The 100 samples collected are brought to laboratory, their
infected root and stems are surfaced-sterilized and placed on Potato Dextrose
Agar (PDA). Fungal colonies are sub-cultured and purified on PDA then they are identified
with pathogenicity tests and light microscopy. There are four races (0, 1, 2,
and 1.2) defined based on their phenotypic reactions with our melon accessions.
The FOM race 1.2 is the most virulent and yield-limiting race found with
causing severe wilting and deaths on infected melon plants. The other three
races are less virulent than race 1.2 resulting several yellowing, necrosis and
wilting on melon cultivars. The identified four races of FOM are long-term
stored in cryotubes for further breeding, marker assisted selection and
biotechnology studies.