The HLA-G 14bp gene polymorphism and decidual HLA-G 14bp gene expression in pre-eclamptic and normal pregnancies

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Iversen A., Nguyen O. T. D., Tommerdal L. F., Eide I. P., Landsem V. M., Acar N., ...More

JOURNAL OF REPRODUCTIVE IMMUNOLOGY, vol.78, no.2, pp.158-165, 2008 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 78 Issue: 2
  • Publication Date: 2008
  • Doi Number: 10.1016/j.jri.2008.03.001
  • Journal Name: JOURNAL OF REPRODUCTIVE IMMUNOLOGY
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.158-165
  • Keywords: decidua, gene polymorphism, HLA-G, pre-eclampsia, pregnancy, G MESSENGER-RNA, G GENOTYPE, EXTRAVILLOUS TROPHOBLASTS, G MOLECULE, CLASS-I, ANTIGEN, CELLS, DELETION, FORM, TRANSCRIPTS
  • Akdeniz University Affiliated: Yes

Abstract

Trophoblast expression of the non-classical MHC, HLA-G, is considered essential for feto-maternal immune tolerance and successful placentation in pregnancy. The HLA-G 14 bp polymorphism in the 3'-untranslated region (UTR) of the HLA-G gene has been reported to be associated with development of pre-eclampsia (PE). In this study, maternal (peripheral blood, n = 54) and fetal (cord blood, n = 57) HLA-G 14 bp genotypes have been determined by PCR in pre-eclamptic and normal pregnancies. In addition, HLA-G 14 bp gene expression in decidua basalis (n = 59) was analyzed by RT-PCR. The pre-eclamptic syndrome was neither associated with the HLA-G 14 bp genotype (maternal or fetal), nor with altered decidual HLA-G 14 bp gene expression. Furthermore, the HLA-G 14 bp mRNA expressed in decidua basalis was of fetal origin and all potential transcripts, as predicted from the fetal HLA-G 14 bp genotype, were expressed. In contrast to previous findings, we found no correlation between the HLA-G 14 bp polymorphism and fetal growth. In conclusion, the fetal HLA-G 14 bp genotype is reflected in the decidual HLA-G mRNA splice form profile, but does not appear to be associated with increased risk for development of PE.