Research Information System
IV International Plant Breeding Congress , Antalya, Turkey, 21 - 25 November 2022, pp.225-232, (Full Text)
While the cultivated eggplant genotypes are susceptible to many biotic and abiotic stress factors the wild
relatives of eggplant can be resistant to more than one factor. Nevertheless, the success in interspecific
cross between cultivated form and wild relatives of eggplant is very limited because of incompatibility.
Hence, using biotechnological approaches such as somatic hybridization can be useful tool to overcome
this problem. This study aimed to achieve protoplast isolation, which is the first step for an alternative
method for creating hybrid plants using haploid protoplasts for fusion that results directly with creation
of a diploid plant. The use of haploid plants as a source of protoplasts is of great importance in terms of
the ploidy level of the hybrids to be obtained in somatic hybridization, and no study has been found in
eggplant on using haploid cells as a source of protoplasts and isolating them. The present study reports the
isolation of viable protoplast from anther-derived haploid leaves of Solanum sisymbriifolium, which is the
wild species, a useful material in eggplant plant improvement. Haploid plant leaves obtained by culturing
anthers of Solanum sisymbriifolium Lam. yielded quality protoplasts after isolation and found to be useful
as a protoplast source for further somatic hybridization studies.