Comparison of human articular chondrocyte and chondroprogenitor cocultures and monocultures: To assess chondrogenic potential and markers of hypertrophy
TISSUE & CELL, vol.57, pp.42-48, 2019 (SCI-Expanded, Scopus)
- Publication Type: Article / Article
- Volume: 57
- Publication Date: 2019
- Doi Number: 10.1016/j.tice.2019.01.007
- Journal Name: TISSUE & CELL
- Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
- Page Numbers: pp.42-48
- Keywords: Cartilage, Chondrocytes, Chondrogenesis, Chondroprogenitors, Co-culture, MESENCHYMAL STEM-CELLS, PROGENITOR CELLS, GENE-EXPRESSION, STROMAL CELLS, BONE-MARROW, OSTEOARTHRITIC CARTILAGE, MONOLAYER-CULTURE, FAT PAD, TISSUE, SURFACE
- Akdeniz University Affiliated: Yes
Abstract
Background and Objective: In the field of cartilage repair, use of two or more cell populations such as mesenchymal stem cells with chondrocytes in an in-vitro co-culture synergistic environment has been attempted to evade limitations of monoculture systems and promote/induce chondrogenesis. Articular cartilage-derived chondroprogenitors (CPs), considered to have stem-cell like characteristics have been proposed as a potential contender for neocartilage development. Our objective was to assess whether co-cultures using different ratios of chondrocytes(C) and CPs would demonstrate better results in terms of growth kinetics, surface marker expression, chondrogenic potential, tendency for hypertrophy and glycosaminoglycan deposition than monocultures.