Comparison of human articular chondrocyte and chondroprogenitor cocultures and monocultures: To assess chondrogenic potential and markers of hypertrophy

Vinod E., Kachroo U., Ozbey O., SATHİSHKUMAR S., Boopalan P. R. J. V. C.

TISSUE & CELL, cilt.57, ss.42-48, 2019 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 57
  • Basım Tarihi: 2019
  • Doi Numarası: 10.1016/j.tice.2019.01.007
  • Dergi Adı: TISSUE & CELL
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.42-48
  • Anahtar Kelimeler: Cartilage, Chondrocytes, Chondrogenesis, Chondroprogenitors, Co-culture, MESENCHYMAL STEM-CELLS, PROGENITOR CELLS, GENE-EXPRESSION, STROMAL CELLS, BONE-MARROW, OSTEOARTHRITIC CARTILAGE, MONOLAYER-CULTURE, FAT PAD, TISSUE, SURFACE
  • Akdeniz Üniversitesi Adresli: Evet

Özet

Background and Objective: In the field of cartilage repair, use of two or more cell populations such as mesenchymal stem cells with chondrocytes in an in-vitro co-culture synergistic environment has been attempted to evade limitations of monoculture systems and promote/induce chondrogenesis. Articular cartilage-derived chondroprogenitors (CPs), considered to have stem-cell like characteristics have been proposed as a potential contender for neocartilage development. Our objective was to assess whether co-cultures using different ratios of chondrocytes(C) and CPs would demonstrate better results in terms of growth kinetics, surface marker expression, chondrogenic potential, tendency for hypertrophy and glycosaminoglycan deposition than monocultures.