Beneficial Effect of Doxazosin on Renal Apoptosis After Partial Bladder Outlet Obstruction

KUTLU Ö., YALCINKAYA M., ELPEK G. Ö., KÖKSAL İ. T., KUKUL E.

TURKIYE KLINIKLERI TIP BILIMLERI DERGISI, cilt.30, sa.5, ss.1575-1580, 2010 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 30 Sayı: 5
  • Basım Tarihi: 2010
  • Doi Numarası: 10.5336/medsci.2009-13934
  • Dergi Adı: TURKIYE KLINIKLERI TIP BILIMLERI DERGISI
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.1575-1580
  • Anahtar Kelimeler: Apoptosis, kidney, doxazosin, BENIGN PROSTATIC HYPERPLASIA, URINARY-TRACT SYMPTOMS, TUBULAR CELL APOPTOSIS, ANGIOTENSIN-II, ALPHA-1-ADRENOCEPTOR ANTAGONISTS, URETERAL OBSTRUCTION, ALPHA-ADRENOCEPTOR, RECEPTOR SUBTYPES, CARCINOMA-CELLS, CANCER CELLS
  • Akdeniz Üniversitesi Adresli: Evet

Özet

Objective: It is not known whether inhibition of alpha 1-adrenoreceptor in a rat model of partial bladder outlet obstruction (BOO) causes decrease in tubular and glomerular cell death by apoptosis and prevention of subsequent inflammation and fibrosis. The aim of this study was to demonstrate the relationship between apoptosis and doxazosin alpha 1-adrenoceptor antagonist) in rat kidneys damaged by partial BOO. Material and Methods: A total of 45 adult female Wistar rats,with a mean weight of 250 g were randomly allocated to four experimental groups as: sham-operated rats (10 rats), sham-operated rats treated with doxazosin (10 rats), partial BOO group (14 rats), and partial BOO group treated with doxazosin (11 rats). Partial BOO was surgically induced. Detection of cell apoptosis was done by TUNEL assay. Results: This investigation revealed increased apoptosis in both tubular and glomerular cells of rats with partial BOO. Furthermore, the results of this study demonstrated that doxazosin treatment of rats reduced apoptosis in tubular and glomerular cells in both the controls and the rats with partial BOO. Conclusion: We believe that our observation of decreased apoptosis in both tubular and glomerular cells of rats with partial BOO might be useful to gain new insight into the therapeutic effect of doxazosin.