Akademik Veri Yönetim Sistemi
IV. INTERNATIONAL PLANT BREEDING CONGRESS, Antalya, Türkiye, 21 - 25 Kasım 2022, ss.89
Eggplant (Solanum melongena L.,) is one of the main used vegetables in many countries. It has a long growth period which makes as one of the vegetables that is more exposed to pests, disease infestation, environmental stresses and parasitic weeds. Orobanche spp. known as broomrape is one of the parasitic weeds that can cause huge damages to yields off eggplant. It is a well- known fact that these parasite seeds for germination need to be exposed to specific chemicals called strigolactones [SLs], which are released by the host roots. The CCD8 gene is involved in the synthesis of strigolactones and plays an important role in controlling growth and development. CRISPR-Cas9 has been applied to manipulate the SlCCD8 gene in eggplant (Solanum melongena). Using the Agrobacterium-mediated plant transformation method gRNA and Cas9 were transferred to eggplant. As the material was used eggplant cotyledons. The successful editing of eggplant lines was confirmed by Sanger sequencing. The induced mutations were stably inherited in the T1 progeny. Our findings based using CRISPR-Cas9 technology opens the way to the development of eggplant genotype’s resistance to parasitic weeds
Keywords: Agrobacterium tumefaciens-mediated,
CCD8, CRISPR-Cas9 technology, Eggplant, Orobanche
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