Integrated biomarker responses to dietary polypropylene microplastics in rainbow trout (Oncorhynchus mykiss) under simulated aquaculture conditions
Aquaculture International, cilt.34, sa.7, 2026 (SCI-Expanded, Scopus)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 34 Sayı: 7
- Basım Tarihi: 2026
- Doi Numarası: 10.1007/s10499-026-02655-3
- Dergi Adı: Aquaculture International
- Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, BIOSIS, Geobase, INSPEC, Zoological Record, Academic Search Ultimate (EBSCO), Natural Science Collection (ProQuest), Earth, Atmospheric, & Aquatic Science Collection (ProQuest)
- Anahtar Kelimeler: Apoptosis biomarkers, Aquatic pollution, Hematological biomarkers, Oxidative damage, Plastic pollution
- Akdeniz Üniversitesi Adresli: Evet
Özet
Microplastics (MPs) are prevalent contaminants in freshwater ecosystems; however, their effects on ecologically and economically important fish species, such as rainbow trout (Oncorhynchus mykiss), remain poorly understood, particularly from an integrated biomarker perspective. This study evaluated the effects of dietary polypropylene microplastic (PP-MPs) exposure on growth performance, hematological parameters, and tissue-specific oxidative stress responses in O. mykiss. Fish were fed with diets containing 0% (control), 10% (10 PP), or 20% (20 PP) PP-MPs (w/w) for 45 days, with responses assessed at 15-day intervals (days 0, 15, 30, and 45). Growth performance (weight gain, feed conversion ratio, and specific growth rate) and hematological parameters (RBC, WBC, HGB, HCT, MCV, MCH, MCHC, and PLT) were evaluated. Oxidative stress, antioxidant defense, DNA damage, and apoptosis were assessed using SOD, CAT, GPx, GR, GSH, ROS, MDA, 8-OHdG, and Cas-3 in the brain, liver, gill, and muscle, while AChE activity was measured in brain tissue as a neurotoxicity biomarker. PP-MPs residues were detected in the gill and gastrointestinal tissues. Dietary PP-MPs exposure reduced growth performance in a dose- and time-dependent manner, while effects on the hepatosomatic index were transient and the viscerosomatic index remained unchanged. Multivariate analyses revealed significant effects of treatment and exposure duration, with an overall response pattern of liver > brain ≈ muscle > gill, indicating systemic but tissue-specific physiological responses. Biomarker responses were not uniformly significant across tissues, and the observed growth impairment may have been influenced by both PP-MPs-related physiological stress and minor dietary nutrient dilution.