Akademik Veri Yönetim Sistemi
Akdeniz Tıp Dergisi, cilt.9, sa.3, ss.277-283, 2023 (Hakemli Dergi)
Objective: Although it is known that there are changes in the vascular purinergic system in diabetes, it is unknown whether P2X1-mediated vascular responses are affected. We aimed to investigate the vascular responses mediated by P2X1 receptor activation in the streptozotocin-induced diabetes model, in this study. Method: Animals were divided into two groups: diabetes and control. Diabetes was induced by 65 mg/kg single dose of streptozotocin. After 12 weeks, second branches of the mesenteric artery were isolated and placed into the wire myograph to evaluate the vascular responses to (ATP) and P2X1 receptor agonist. Vascular responses were also examined in the presence of endothelial nitric oxide synthase, cyclooxygenase, or K+ channel inhibitors, to determine the possible mechanism/s of relaxation responses. Results: In the diabetes group relaxation responses to ATP and P2X1 receptor agonists were lower compared to the control group. Vascular relaxation responses to P2X1 receptor agonists were significantly decreased in both groups in the presence of endothelial nitric oxide synthase inhibitor. Cyclooxygenase inhibitor and K+ channels inhibitors significantly blocked vascular relaxation responses in the diabetes group but not in control animals. Conclusion: The results of this study revealed that vascular P2X1 receptor-mediated relaxation responses are decreased in diabetes and the pathways mediating these responses were changed.