Electrochemical Tau Protein Immunosensor Based on MnS/GO/PANI and Magnetite-incorporated Gold Nanoparticles

Yola B. B., KARAMAN C., Özcan N., Atar N., Polat İ., Yola M. L.

Electroanalysis, cilt.34, sa.9, ss.1519-1528, 2022 (SCI-Expanded) identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 34 Sayı: 9
  • Basım Tarihi: 2022
  • Doi Numarası: 10.1002/elan.202200159
  • Dergi Adı: Electroanalysis
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, Aerospace Database, Applied Science & Technology Source, BIOSIS, Chemical Abstracts Core, Chimica, Communication Abstracts, Metadex, Civil Engineering Abstracts
  • Sayfa Sayıları: ss.1519-1528
  • Anahtar Kelimeler: Alzheimer's disease, Detection, Diagnosis, Nanocomposite, Voltammetry
  • Akdeniz Üniversitesi Adresli: Evet

Özet

© 2022 Wiley-VCH GmbH.Herein, electrochemical tau protein immunosensor based on manganese sulfide nanoparticles/graphene oxide/polyaniline (MnS/GO/PANI) and magnetite-incorporated gold nanoparticles (AuNPs@Fe3O4) was constructed. After the modification of the glassy carbon electrode (GCE) with MnS/GO/PANI, the immobilization and conjugation of anti-Tau capture antibody and antigen Tau protein were successfully completed on MnS/GO/PANI/GCE, respectively. Then, the conjugation of anti-Tau secondary antibody was carried out to AuNPs@Fe3O4 as signal amplification via amino-gold affinity and the eventual immunosensor was accomplished by the distinctive interactions of electrode platform and signal amplification. Final immunosensor demonstrated the quantification limit (LOQ) of 1.0×10−13 M and the detection limit (LOD) of 1.0×10−14 M.