Akademik Veri Yönetim Sistemi
4th International Congress on Plant Biology, Denizli, Türkiye, 3 - 06 Eylül 2025, ss.131, (Özet Bildiri)
Nickel is an essential trace element for plants; however, at elevated concentrations, it induces
toxic effects. Phytoremediation, offers an effective strategy for mitigating nickel contamination.
One approach to enhance the efficiency of this technique involves the use of plant growth-
promoting bacteria, which can improve metal accumulation and tolerance in plants. In this
study, the phytoremediation potential of canola (Brassica napus L. cv. Samibey) exposed to
nickel stress was investigated in the presence of the rhizospheric bacterium Pseudomonas
thivervalensis. Throughout the 24-day experimental period, seedlings were grown under
controlled conditions: 25 °C temperature, 50% humidity, a 16-hour light/8-hour dark
photoperiod, and a light intensity of 200–250 µmol m⁻² s⁻¹, using Hoagland’s nutrient solution.
P. thivervalensis-inoculated and non-inoculated groups were exposed to 0.25 mM and 0.50 mM
concentrations of nickel during the last 6 days of the experiment. Based on the findings, nickel
predominantly accumulated in the root tissues; however, bacterial inoculation enhanced its
translocation to the shoots by 56%. Nickel stress decreased relative water content, chlorophyll
a levels, and biomass by approximately 25%, 45%, and 50%, respectively, while P.
thivervalensis inoculation led to a 20% increase in chlorophyll a content. In addition, bacterial
inoculation significantly reduced electrolyte leakage by 28.5% and enhanced photosynthetic
efficiency by 61.2%, compared to non-inoculated, nickel-stressed plants. Furthermore,
although nickel stress increased antioxidant enzyme activities, inoculation with P.
thivervalensis resulted in reductions of up to 27.2% in superoxide dismutase, 23.5% in guaiacol
peroxidase, and 17.5% in glutathione reductase activities relative to stressed control plants. In
conclusion, canola co-treated with P. thivervalensis demonstrated strong potential for nickel
phytoextraction and tolerance, highlighting the synergistic role of plant–microbe interactions
in phytoremediation applications.
Keywords: Antioxidant, Chlorophyll a Fluorescence, PGPB, Phytoremediation
Acknowledgments: This study was supported by Akdeniz University Scientific Research
Projects Coordination Unit (Project No: FKA1-2022-5932).