Observing better transfection efficiency in primary cultured VSMCs: Comparison and development of two different protocols

TOKAY A., ÇETİN A., Uzuner F., YEŞİLKAYA A.

TURKISH JOURNAL OF BIOCHEMISTRY-TURK BIYOKIMYA DERGISI, cilt.37, sa.1, ss.94-98, 2012 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 37 Sayı: 1
  • Basım Tarihi: 2012
  • Doi Numarası: 10.5505/tjb.2012.65375
  • Dergi Adı: TURKISH JOURNAL OF BIOCHEMISTRY-TURK BIYOKIMYA DERGISI
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, TR DİZİN (ULAKBİM)
  • Sayfa Sayıları: ss.94-98
  • Anahtar Kelimeler: Transfection, FuGENE, Lipofectamine, pCH110, VSMCs, DNA TRANSFER, CELLS
  • Akdeniz Üniversitesi Adresli: Evet

Özet

Aim: In this study, we have evaluated the procedure of transient transfection efficiency of vascular smooth muscle cells (VSMCs) with Lipofectamine (Life Technologies) and FuGENE (ROCHE, FuGENE HD Reagent) transfection reagents using the pCH110 eukaryotic assay vector containing the lacZ reporter gene. We also did try these attainments to transfect VSMCs with Ras(N17) DNA and affirmed our findings.