Observing better transfection efficiency in primary cultured VSMCs: Comparison and development of two different protocols
TURKISH JOURNAL OF BIOCHEMISTRY-TURK BIYOKIMYA DERGISI, vol.37, no.1, pp.94-98, 2012 (SCI-Expanded, Scopus, TRDizin)
- Publication Type: Article / Article
- Volume: 37 Issue: 1
- Publication Date: 2012
- Doi Number: 10.5505/tjb.2012.65375
- Journal Name: TURKISH JOURNAL OF BIOCHEMISTRY-TURK BIYOKIMYA DERGISI
- Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus, TR DİZİN (ULAKBİM)
- Page Numbers: pp.94-98
- Keywords: Transfection, FuGENE, Lipofectamine, pCH110, VSMCs, DNA TRANSFER, CELLS
- Akdeniz University Affiliated: Yes
Abstract
Aim: In this study, we have evaluated the procedure of transient transfection efficiency of vascular smooth muscle cells (VSMCs) with Lipofectamine (Life Technologies) and FuGENE (ROCHE, FuGENE HD Reagent) transfection reagents using the pCH110 eukaryotic assay vector containing the lacZ reporter gene. We also did try these attainments to transfect VSMCs with Ras(N17) DNA and affirmed our findings.