Discrimination of Aspergillus flavus from Aspergillus oryzae by matrix-assisted laser desorption/ionisation time-of-flight (MALDI-TOF) mass spectrometry

HEDAYATI M. T., TAGHIZADEH-ARMAKI M., ZARRINFAR H., HOSEINNEJAD A., ANSARI S., ABASTABAR M., ...Daha Fazla

MYCOSES, cilt.62, sa.12, ss.1182-1188, 2019 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 62 Sayı: 12
  • Basım Tarihi: 2019
  • Doi Numarası: 10.1111/myc.13010
  • Dergi Adı: MYCOSES
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.1182-1188
  • Anahtar Kelimeler: Aspergillus flavus, Aspergillus oryzae, identification, MALDI-TOF MS, beta-tubulin, DESORPTION IONIZATION-TIME, RAPID IDENTIFICATION, FUSARIUM, MS, DIFFERENTIATION, MUCORALES, BIOTYPER, MOLDS, SPP., TOOL
  • Akdeniz Üniversitesi Adresli: Evet

Özet

Background Aspergillus flavus is a major cause of severe non-invasive fungal infections in the Middle Eastern countries. However, it is difficult to distinguish A flavus from A oryzae. Objectives To assess the potential of matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry (MALDI-TOF MS) in discriminating between A flavus and A oryzae and compare it with beta-tubulin gene sequencing. Methods We used the Bruker Daltonik MALDI-TOF MS system to analyse 200 clinical and environmental A flavus isolates and one A pseudonomius and one A alliaceus (Aspergillus section Flavi) isolate a priori identified as such by sequencing of the beta-tubulin gene. Results All 200 A flavus isolates were identified at the genus level and 176 (88%) at the species levels by MALDI-TOF MS based on the spectral log-scores (>= 2.0 and 1.7-1.99, respectively); among them, only 18 (10.2%) were confirmed as A flavus, whereas 35 (19.9%) were identified as A oryzae and 123 (69.9%) as A flavus/A oryzae. Aspergillus pseudonomius and A alliaceus were misidentified as A flavus and A parasiticus with log-score values of 1.39 and 1.09, respectively. Conclusions The results indicate that the commercially available Bruker Daltonik MALDI-TOF MS score database cannot separate A flavus and A oryzae species. We also showed that establishment of an in-house library is a useful tool to discriminate closely related Aspergillus species, including A flavus and A oryzae.