The effect of hemolysis on plasma oxidation and nitration in patients with sickle cell disease

Kupesiz A., Celmeli G., Dogan S., Antmen B., Aslan M.

FREE RADICAL RESEARCH, cilt.46, sa.7, ss.883-890, 2012 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 46 Sayı: 7
  • Basım Tarihi: 2012
  • Doi Numarası: 10.3109/10715762.2012.686037
  • Dergi Adı: FREE RADICAL RESEARCH
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.883-890
  • Anahtar Kelimeler: sickle cell disease, haemolysis, nitric oxide, plasma, oxidation, ACUTE CHEST SYNDROME, NITRIC-OXIDE, PULMONARY-HYPERTENSION, FREE HEMOGLOBIN, MICROVASCULAR DYSFUNCTION, CLINICAL-SIGNIFICANCE, VASCULAR FUNCTION, ARGININE THERAPY, RISK-FACTORS, ANEMIA
  • Akdeniz Üniversitesi Adresli: Evet

Özet

The effect of hemolysis on plasma oxidation and nitration in patients with sickle cell disease.

Kupesiz A1, Celmeli G, Dogan S, Antmen B, Aslan M.

Abstract

This study aimed to determine the effect of haemolysis on plasma oxidation and nitration in sickle cell disease (SCD) patients. Blood was collected from haemoglobin (Hb)A volunteers and homozygous HbSS patients who had not received blood transfusions in the last 3 months. Haemolysis was characterised by low levels of haemoglobin and haptoglobin and high levels of reticulocyte, mean corpuscular volume (MCV), mean corpuscular haemoglobin (MCH), plasma cell-free haemoglobin, bilirubin, total lactate dehydrogenase (LDH) and dominance of LDH-1 isoenzyme. Plasma 8-isoprostane, protein carbonyl and nitrotyrosine levels were measured to evaluate oxidised lipids, oxidised and nitrated proteins, respectively. Plasma nitrite-nitrate levels were also determined to assess nitric oxide (NO) production in both SCD patients and controls. Markers of haemolysis were significantly evident in SCD patients compared to controls. Plasma 8-isoprostane, protein carbonyl and nitrotyrosine levels were markedly elevated in SCD patients compared to controls. Linear regression analysis revealed a significant inverse correlation between haemoglobin and reticulocyte counts and a significant positive correlation of plasma cell-free haemoglobin with protein carbonyl and nitrotyrosine levels. The obtained data shows that increased haemolysis in SCD increases plasma protein oxidation and nitration.